Protein Info for HSERO_RS02855 in Herbaspirillum seropedicae SmR1

Annotation: D-aminoacylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 PF07969: Amidohydro_3" amino acids 51 to 233 (183 residues), 63.3 bits, see alignment E=4.9e-21 amino acids 334 to 463 (130 residues), 53 bits, see alignment E=6.5e-18 PF01979: Amidohydro_1" amino acids 57 to 461 (405 residues), 46.7 bits, see alignment E=4e-16

Best Hits

Swiss-Prot: 60% identical to NDAD_ALCXX: D-aminoacylase (dan) from Alcaligenes xylosoxydans xylosoxydans

KEGG orthology group: K06015, N-acyl-D-amino-acid deacylase [EC: 3.5.1.81] (inferred from 100% identity to hse:Hsero_0570)

Predicted SEED Role

"D-aminoacylase (EC 3.5.1.81)" (EC 3.5.1.81)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.81

Use Curated BLAST to search for 3.5.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IYC9 at UniProt or InterPro

Protein Sequence (498 amino acids)

>HSERO_RS02855 D-aminoacylase (Herbaspirillum seropedicae SmR1)
MQNSPAFDLLITGGTIIDGSKAPRFRADIGVRHGRIAEIGNLSHRQAAQRIDASGKIVAP
GFIDSHTHDDQAVLSQPDMTFKISQGVTTVITGNCGISIAPLRAGTPLPAPLSLLDDADG
TRFETFSSYLDALRQTPSAVNVAAMVGHSTLRVMTMDALEREARPDEISAMNALLDEALE
AGAIGISTGTYYPPAAHASTQEVIDVCRPIAGTGALYVTHMRDEADKSMEALEESFLIGR
ELNAPVVISHHKLQRAHNFGKSSQTLARIRQAMQCQCVALDCYPYNASSTMLHRDPKRLV
GRILISSSKPHPEQAGRELDAIAAEWGVSKEEAAARLQPASAVYFGMDESDVRNILAFDE
TMIGSDGIPTGEKPHPRLWGTFPRILGHYSRDLGLFDLEVAVWKMTGLTARNFKLHERGT
LEVGHYADIVVFDAATIRDTASYETPAEPAQGIDAVIVNGVVTWRLGQHMGARAGHVIAR
HAASKRRDQAPCRSKASS