Protein Info for HSERO_RS02410 in Herbaspirillum seropedicae SmR1

Annotation: acriflavine resistance protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1048 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 387 (22 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 441 to 466 (26 residues), see Phobius details amino acids 473 to 497 (25 residues), see Phobius details amino acids 540 to 558 (19 residues), see Phobius details amino acids 869 to 887 (19 residues), see Phobius details amino acids 894 to 914 (21 residues), see Phobius details amino acids 921 to 943 (23 residues), see Phobius details amino acids 968 to 989 (22 residues), see Phobius details amino acids 1001 to 1023 (23 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1039 (1039 residues), 1620.4 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1024 (1024 residues), 1258.8 bits, see alignment E=0 PF03176: MMPL" amino acids 338 to 499 (162 residues), 31.3 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 58% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 66% identity to adk:Alide2_0399)

MetaCyc: 56% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IXC8 at UniProt or InterPro

Protein Sequence (1048 amino acids)

>HSERO_RS02410 acriflavine resistance protein B (Herbaspirillum seropedicae SmR1)
MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS
VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA
AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT
ERAMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNGQL
TSPEQFGNIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL
GTATAVHKKMEELAKYFPTGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQ
NIRYTIIPTIVVPVALLGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIVVVENVERIM
SEEGLSPADATRKAMGQISGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSLSMVASMVF
SVLMAFTLTPALCATILKPVEKGHHHEKKGFFGWFNRKFNSTASGYQGIIGGMLNKAGRY
MIVYVLIVACVGLLYVRLPASFLPNEDQGYIITNVQLPPGASTSRTTAVVKQIEEYYLKD
PAVEHAIAVLGYSFSGNGQNAALVFTPLKDWSQRDKSQSADAIAGKAFGAFSQIKDAIVF
PLNPPPIPELGNATGFTFRLQDRAGLGHTALVQARNQILGMASQSKVLAGVRPEGLEDAP
QLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNLGRQQRVIVQADAKRRLQPED
LTNLYARNSSGTMVPFSAFVTAKWINGPVQLIRYNGYPAMKIAGGAAPGRSTGEAMAEME
SFVAKLPPGFGFEWTGQSLEEKTSGNQAPALYALSLIAVFLVLAALYESTSIPLAVMLVV
PLGILGALLGVTLRAMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGKGLIEATLE
AVHLRFRPIIMTSLAFILGVLPLAIASGAGSASQRAIGTGVMGGMITATVLAVFLVPIFF
VVVRKIFKGSERQRKFDAEHSPKINSGE