Protein Info for HSERO_RS02310 in Herbaspirillum seropedicae SmR1

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01979: Amidohydro_1
  • amino acids 57 to 421 (365 residues), 150 bits, E=1.1e-47
• PF07969: Amidohydro_3
  • amino acids 105 to 442 (338 residues), 37 bits, E=3.1e-13

Best Hits

Swiss-Prot: 68% identical to IXPDE_UNKP: Isoxanthopterin deaminase from Unknown prokaryotic organism

KEGG orthology group: K03382, hydroxyatrazine ethylaminohydrolase [EC: 3.5.99.3] (inferred from 100% identity to hse:Hsero_0463)

Predicted SEED Role

"BOX elements"

KEGG Metabolic Maps

• Atrazine degradation

Isozymes

Compare fitness of predicted isozymes for: 3.5.99.3

Use Curated BLAST to search for 3.5.99.3

Sequence Analysis Tools

See D8IXB3 at UniProt or InterPro

Protein Sequence (471 amino acids)

>HSERO_RS02310 amidohydrolase (Herbaspirillum seropedicae SmR1)
MSTFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGEELIDASHCVAYPA
WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSGC
GTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALRPETLD
GFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREMGLRLHSHLSE
TVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSN
GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRG
GHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPA
IGPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQAVKKMLKSN