Protein Info for HSERO_RS02115 in Herbaspirillum seropedicae SmR1

Annotation: valyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 936 transmembrane" amino acids 485 to 504 (20 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 4 to 917 (914 residues), 1121.2 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 17 to 612 (596 residues), 475.5 bits, see alignment E=3.3e-146 PF09334: tRNA-synt_1g" amino acids 44 to 186 (143 residues), 28.9 bits, see alignment E=1.3e-10 amino acids 373 to 437 (65 residues), 20.7 bits, see alignment 4e-08 PF13603: tRNA-synt_1_2" amino acids 241 to 300 (60 residues), 27.7 bits, see alignment 4.7e-10 PF08264: Anticodon_1" amino acids 663 to 816 (154 residues), 124.7 bits, see alignment E=7.1e-40 PF10458: Val_tRNA-synt_C" amino acids 871 to 936 (66 residues), 75.7 bits, see alignment 7e-25

Best Hits

Swiss-Prot: 76% identical to SYV_HERAR: Valine--tRNA ligase (valS) from Herminiimonas arsenicoxydans

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 69% identity to ajs:Ajs_2896)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IX76 at UniProt or InterPro

Protein Sequence (936 amino acids)

>HSERO_RS02115 valyl-tRNA synthetase (Herbaspirillum seropedicae SmR1)
MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI
MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE
KSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVL
GTAVSDLEVVSEEEDGSMWHIKYPLADGSGHIVVATTRPETMLGDVAVAVDPTDERYAAL
VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA
TINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTGVVIEPMLTDQW
FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW
WGHQIPAWYDEDGGIYVARTEEEARAQAGGRAVKRDEDVLDTWFSSALVPFSTLGWPEET
PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK
GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA
SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFS
KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ
QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSIMRQAYPRPDLE
KIDAEAEAWVAQFKAYTDACRNLRGEMQLAPSLRVPLIVEAADASAVESFLPYLQGLVRL
SETTVVAQLPESPAPVAIVGEVKLMLKVEIDVAAERERLGKEVTRLTAEIAKANGKLSNE
SFVARAPEAVVAQEKERVANFSATLEKVKEQLAKLK