Protein Info for HSERO_RS01990 in Herbaspirillum seropedicae SmR1

Annotation: shikimate 5-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00507: shikimate dehydrogenase
  • amino acids 4 to 269 (266 residues), 270.2 bits, E=7.9e-85
• PF08501: Shikimate_dh_N
  • amino acids 6 to 87 (82 residues), 85.9 bits, E=2.6e-28
• PF01488: Shikimate_DH
  • amino acids 115 to 191 (77 residues), 40.5 bits, E=4.2e-14
• PF18317: SDH_C
  • amino acids 236 to 259 (24 residues), 36 bits, see alignment (E = 6.8e-13)

Best Hits

Swiss-Prot: 64% identical to AROE_JANMA: Shikimate dehydrogenase (NADP(+)) (aroE) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K00014, shikimate dehydrogenase [EC: 1.1.1.25] (inferred from 100% identity to hse:Hsero_0401)

Predicted SEED Role

"Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 1.1.1.25)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.25

Use Curated BLAST to search for 1.1.1.25

Sequence Analysis Tools

See D8IX51 at UniProt or InterPro

Protein Sequence (271 amino acids)

>HSERO_RS01990 shikimate 5-dehydrogenase (Herbaspirillum seropedicae SmR1)
MDEYVVIGNPIAHSKSPDIHARFAAQTGQSMSYERLLVPLHGFKAAVQVFQRRGGRGANI
TVPFKLEAHALADQLTERARLAGAVNTFKFEEGCILGDNTDGAGLVTDIMVNAGQALQGR
RILLLGAGGASRGALLPLLQQRPAELVVVNRTASRAEELATQFAAYGPVRSTTFAGLEGA
FDVIVNATSASLSDDLPPLPPSVYGSETLAYDMMYGRGPTVFMQSAQQHGAHVRDGLGML
VEQAAEAFQWWRGVRPATAEVFASLRQQLSQ