Protein Info for HSERO_RS01830 in Herbaspirillum seropedicae SmR1

Annotation: prolyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 568 (568 residues), 743.6 bits, E=9e-228
• PF00587: tRNA-synt_2b
  • amino acids 95 to 462 (368 residues), 118.5 bits, E=5.4e-38
• PF04073: tRNA_edit
  • amino acids 257 to 381 (125 residues), 99.7 bits, E=1.9e-32
• PF03129: HGTP_anticodon
  • amino acids 479 to 570 (92 residues), 70.4 bits, E=1.8e-23

Best Hits

Swiss-Prot: 83% identical to SYP_JANMA: Proline--tRNA ligase (proS) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to hse:Hsero_0370)

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.15

Sequence Analysis Tools

See D8IW97 at UniProt or InterPro

Protein Sequence (575 amino acids)

>HSERO_RS01830 prolyl-tRNA synthetase (Herbaspirillum seropedicae SmR1)
MRASRFFISTLKEAPSDAEIVSHKLMMRAGMIKRLGAGIYTYMPMGLRVIRKVEAIVREE
MNRAGAVELLMPVVQPAELWQETGRWNKMGAELMRVKDRHGRDFAIQPTSEEVVTDVARS
ELRSYKQLPVNFYHIQTKFRDERRPRFGLMRGREFTMKDAYSFDRDVDGLKKSYQAMYDA
YVRIFTRFGLEFRAVAADNGAIGGSGSHEFHVVAATGEDALVYCPTSDYAANMEAAEALP
VDSTRGAATQALEKTATPGKSKCEAVAELLGLPLARTVKSIVLAVDNEDPAKKEIWLLLL
RGDHELNEVKASKIPGLSTYRFANEAEIVEWFGTPPGYLGPIGTKKPVKVVADRTVANMA
DFVCGANEADFHYTGANWGRDLPEPLVADIRNVVEGDASPDGKGVLAIQRGIEVGHVFQL
GTTYSEAMKATYLDEAGKPQPLVMGCYGIGVTRILGAAIEQNFDDRGIIWPTSIAPFEVV
LCPMGYDRSEAVKTEIDKLYAQLSEAGVDVVLDDRGERPGAMFADWELIGVPHRIVVGDR
GLKDGNIEYQGRRDTEATQVPLAEALAFIQGKLAA