Protein Info for HSERO_RS01205 in Herbaspirillum seropedicae SmR1
Annotation: 2-hydroxy-acid oxidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 45% identical to MDLB_PSEPU: (S)-mandelate dehydrogenase (mdlB) from Pseudomonas putida
KEGG orthology group: K15054, (S)-mandelate dehydrogenase [EC: 1.1.99.31] (inferred from 100% identity to hse:Hsero_0244)MetaCyc: 45% identical to (S)-mandelate dehydrogenase (Pseudomonas putida)
(S)-mandelate dehydrogenase. [EC: 1.1.99.31]; 1.1.5.- [EC: 1.1.99.31]
Predicted SEED Role
"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)
MetaCyc Pathways
- mandelate degradation to acetyl-CoA (13/18 steps found)
- methylglyoxal degradation V (2/3 steps found)
- mandelate degradation I (3/5 steps found)
- 4-hydroxymandelate degradation (3/6 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (4/8 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (14/42 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.2.3
Use Curated BLAST to search for 1.1.2.3 or 1.1.99.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See D8IV51 at UniProt or InterPro
Protein Sequence (393 amino acids)
>HSERO_RS01205 2-hydroxy-acid oxidase (Herbaspirillum seropedicae SmR1) LKRRLYAGADVQRAQSIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLP RTLVDVSVRRQGRRLFGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVS TTSLEEVVRRSGAQVWQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDAR NFSSPRRLDWRNKLDVLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQS LSAALDWADVQWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTM DVLPEVVAAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRA LEILRSEVDRVLALLACPDIDQLDASYLRRLQA