Protein Info for HSERO_RS00970 in Herbaspirillum seropedicae SmR1

Annotation: glucoamylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 629 PF19291: TREH_N" amino acids 44 to 191 (148 residues), 28.6 bits, see alignment E=8.4e-11 PF00723: Glyco_hydro_15" amino acids 255 to 618 (364 residues), 166.7 bits, see alignment E=8.7e-53

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_0195)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IV02 at UniProt or InterPro

Protein Sequence (629 amino acids)

>HSERO_RS00970 glucoamylase (Herbaspirillum seropedicae SmR1)
MSQPSPDPQQEAQPQPHSQTQHSEQPHLAAQAHDMPSTSSLELGVIGNCAFSALVDKMGR
IVWCCLPRFDGDPVFNALLDPTDNGALWAFQLENFSHSEQWYEPNTAVLRTRLYDTLGQG
IEITDLAPRFQSRGRFFRPLQLVRRIHPLSGAPRMRVVLRPKFDWGREEPLQTRGSNHIR
YVGQEQTLRLNTDAPISYIVGETAFVVSRPYNFILGPDETLHGGINDTARDFEQETTAYW
RNWSRALATPLEWQDAVIRAAITLKLSVYEDTGAIIAAMTTSVPEAPGSQRNWDYRYCWL
RDAFFVVRALNSLSELGTMEDYLRWLTNVVVRSGGGHVQPLYGIGLEEALPEQILDHLPG
YQHHQPVRVGNQAYEHFQHDVYGNIVLGAAQAFHDMRLHHRAGAEHFQHLEAVGERAYEV
YSQPDAGMWELRTRSRIHTSSALMSWAACDRLAKIAARVDLPERARYWGDRAKEIGDRIL
TEAWNEQRQAYAESFGGRDLDASVLLMVEVGLISPTDPRFISTVEALEKSLCDGPYMRRY
EAPDDFGRPETAFNICTFWRIDALARIGRKEQAREIFQAMLDARNHLGLLSEDTHPVTRQ
MWGNFPQTYSMVGIINCAMRLSASWEAVI