Protein Info for HSERO_RS00915 in Herbaspirillum seropedicae SmR1

Annotation: ATP-dependent protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR00390: ATP-dependent protease HslVU, ATPase subunit" amino acids 3 to 448 (446 residues), 680 bits, see alignment E=7.6e-209 PF07728: AAA_5" amino acids 51 to 87 (37 residues), 22.5 bits, see alignment 2.5e-08 PF00004: AAA" amino acids 52 to 97 (46 residues), 28.1 bits, see alignment 6.5e-10 amino acids 242 to 338 (97 residues), 33.1 bits, see alignment E=1.8e-11 PF07724: AAA_2" amino acids 189 to 334 (146 residues), 103.2 bits, see alignment E=3.9e-33

Best Hits

Swiss-Prot: 77% identical to HSLU_CUPNH: ATP-dependent protease ATPase subunit HslU (hslU) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K03667, ATP-dependent HslUV protease ATP-binding subunit HslU (inferred from 100% identity to hse:Hsero_0183)

MetaCyc: 68% identical to ATPase component of the HslVU protease (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent hsl protease ATP-binding subunit HslU" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IUZ0 at UniProt or InterPro

Protein Sequence (448 amino acids)

>HSERO_RS00915 ATP-dependent protease (Herbaspirillum seropedicae SmR1)
MNMTPKEIVSELDKHVVGQARAKRAVSIALRNRWRRQQIEEPLRHEITPKNILMIGPTGV
GKTEIARRLAKLADAPFIKIEATKFTEVGYVGRDVDTIIRDLVDIGIKQTRELETRKVRT
RAEDAAEDRILDVLLPPARDFGFTPDGSNNSGEKDDKNTTRQTFRKRLREGALDDREIEL
ELAEAAPSMEIMAPPGMEEMTEQIKSMFSGLGNQRKKARKIKIKEAMKLLIEEEAAKLVN
EEELKQKAIANVEQNGIVFLDEIDKIATRSQNGNADVSRAGVQRDLLPLVEGTTVNTKYG
MIKTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELDSLSIADFERILTGTDACLTKQY
EALLATEGVKLEFADSGIKRLAEISHSVNEKTENIGARRLYTVMEKLLEEISFTAGEAGV
EGLLIDADYVNERLGELAVDEDLSRYVL