Protein Info for HSERO_RS00435 in Herbaspirillum seropedicae SmR1

Annotation: glutathione synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 TIGR01380: glutathione synthase" amino acids 1 to 311 (311 residues), 421.3 bits, see alignment E=1e-130 PF02951: GSH-S_N" amino acids 1 to 119 (119 residues), 149.5 bits, see alignment E=6.3e-48 PF02955: GSH-S_ATP" amino acids 123 to 296 (174 residues), 240.9 bits, see alignment E=8.6e-76 PF08443: RimK" amino acids 154 to 307 (154 residues), 30.4 bits, see alignment E=4.5e-11

Best Hits

Swiss-Prot: 70% identical to GSHB_RALSO: Glutathione synthetase (gshB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 100% identity to hse:Hsero_0087)

MetaCyc: 49% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ITX6 at UniProt or InterPro

Protein Sequence (316 amino acids)

>HSERO_RS00435 glutathione synthetase (Herbaspirillum seropedicae SmR1)
MKIAFLADPLDTFKTYKDSTFAMMREAARRGHVLYAFEQADLVLESGLVTANACKLTLTG
DADDWYRTDKPVSSFLSEFDAVIVRKDPPFDMEYIYTTYLLELAEKQGARVFNKPAAIRS
HNEKLAIAQFPQYTAPTLVTRDAKRLRAFHAEHQDVILKPLDGMGGMGIFRVKADGMNLG
SIIETLTDNGQRTIMAQRFIPQISAGDKRILLIGGKVVPNVLARIPQGNEVRGNLAAGGL
GRAQPASARDIEIGEALGPVLHERGLLLVGLDAIGDYLTEINVTSPTCFQEITQQTGFDV
AAMFVDALEAEVGSIG