Protein Info for HMPREF1078_RS14965 in Parabacteroides merdae CL09T00C40

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1141 TIGR00392: isoleucine--tRNA ligase" amino acids 8 to 940 (933 residues), 1041.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 20 to 728 (709 residues), 389.4 bits, see alignment E=3.7e-120 PF09334: tRNA-synt_1g" amino acids 52 to 219 (168 residues), 24.4 bits, see alignment E=2.5e-09 amino acids 658 to 733 (76 residues), 23.4 bits, see alignment 4.8e-09 PF08264: Anticodon_1" amino acids 776 to 928 (153 residues), 107.5 bits, see alignment E=1.1e-34 PF19302: DUF5915" amino acids 947 to 1140 (194 residues), 194.2 bits, see alignment E=3.6e-61

Best Hits

Swiss-Prot: 78% identical to SYI_BACFN: Isoleucine--tRNA ligase (ileS) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 78% identity to bfs:BF2367)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1141 amino acids)

>HMPREF1078_RS14965 isoleucine--tRNA ligase (Parabacteroides merdae CL09T00C40)
MSKKFAEYSKLDLSNVNKEVLKKWQDGDIFHKSLEIREGHPSFVFYEGPPSANGMPGIHH
VIARSIKDIFCRYKTMKGYLVNRKAGWDTHGLPVELGVEKTLGITKEDIGKKISVAEYNA
ACRRDVMKFTKEWTDLTQKMGYWVDLENPYITYDNRYIETLWYLLKELYKKGLLYKGYTI
QPYSPAAGTGLSSHELNQPGCYRDVKDTTCTAQFRILDPKPEMAGFGEPFFLAWTTTPWT
LPSNTALCVGPNFTYVAVQTYNPYTGMPMTAVLAKDLLNVYFNPKAADLALTDYKPGDKL
VPFKVVGEWKGPELAGMHYEQLIPWVNPGEGAFRVITGDYVTVEDGTGIVHIAPTFGADD
DRVAKASGVPPLMMIDKDGNRRPMVDMTGKFYLLEDLDPEYVQEHMNAADYDPWQGKFVK
NAYDATKGEKDETLDVEICMMLKAQNRVFRIEKHVHNYPHCWRTDKPVLYYPLDSWFIRT
TACRERMIELNNTINWKPQSTGTGRFGKWLENLQDWNLSRSRYWGTPLPIWRTEDGAEEI
CIGSVEELYNEIEKSVKAGLMESNPYKELKFQPGEYTKENYEKIDLHRPYVDDVILVSES
GKPMKRETDLIDVWFDSGAMPYAQIHYPFENKEIFDDRKVYPADFIAEGVDQTRGWFFTL
HAIATMVFDSVSYKAVVSNGLVLDKNGNKMSKRLGNAVDPFATIEQYGSDPLRWYMITNA
SPWDNIKFDIDGIEEVRRKFFGTLYNTYSFFALYANVDGFDYSEPDVDWKERPEIDRWIL
SLLNSLVKDVDGFLDTYEPTRAGRAISDFVNDNLSNWYVRLNRRRFWGGGMTTDKLSAYQ
TLYTCLETVAKLMAPIAPFYADRLFCDLIAATGREKVESVHLSEFPVCNEAMIDKDLEER
MQMAQDVSSMVLALRRKVNIKVRQPLQTIMVPVVDAHQQESIEAVKALILNEVNVKELKF
VDNAAGILVKKIKPDFKKLGPRYGKIMKALAAAIQAMSQDEINAFEKAGTFTLTFEGQEA
MIERADVEIISEDIPGWLVANEGRLTVALDITVTENLRKEGLARELVNRIQNLRKSSGYD
ITDKISVTVLSNDGMDEAIKDFNSYIANQVLAVSVEITDVISDATEMDFEDFKLSVRIEK
A