Protein Info for HMPREF1078_RS10980 in Parabacteroides merdae CL09T00C40

Annotation: formate-dependent phosphoribosylglycinamide formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF22660: RS_preATP-grasp-like
  • amino acids 14 to 107 (94 residues), 42.5 bits, E=2.1e-14
• TIGR01142: phosphoribosylglycinamide formyltransferase 2
  • amino acids 14 to 392 (379 residues), 588.9 bits, E=2e-181
• PF02222: ATP-grasp
  • amino acids 121 to 300 (180 residues), 196.3 bits, E=1.1e-61
• PF02786: CPSase_L_D2
  • amino acids 121 to 199 (79 residues), 25.8 bits, E=2.2e-09
• PF07478: Dala_Dala_lig_C
  • amino acids 124 to 285 (162 residues), 27.4 bits, E=7.1e-10
• PF21244: PurT_C
  • amino acids 321 to 381 (61 residues), 63.6 bits, E=2.8e-21

Best Hits

Swiss-Prot: 93% identical to PURT_PARD8: Formate-dependent phosphoribosylglycinamide formyltransferase (purT) from Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

KEGG orthology group: K08289, phosphoribosylglycinamide formyltransferase 2 [EC: 2.1.2.2] (inferred from 93% identity to pdi:BDI_3578)

MetaCyc: 63% identical to phosphoribosylglycinamide formyltransferase 2 (Escherichia coli K-12 substr. MG1655)
GARTRANSFORMYL2-RXN [EC: 6.3.1.21]; Acetate kinase. [EC: 6.3.1.21, 2.7.2.1, 2.7.2.15]

Predicted SEED Role

"Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (38/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (18/21 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (20/26 steps found)
• Bifidobacterium shunt (12/15 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• pyruvate fermentation to acetate I (3/3 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to acetate VII (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• acetate and ATP formation from acetyl-CoA I (2/2 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• pyruvate fermentation to acetate II (2/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (9/13 steps found)
• glycine degradation (reductive Stickland reaction) (1/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (5/8 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (2/4 steps found)
• (S)-propane-1,2-diol degradation (2/5 steps found)
• acetylene degradation (anaerobic) (2/5 steps found)
• ethanolamine utilization (2/5 steps found)
• methanogenesis from acetate (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• acetyl-CoA fermentation to butanoate (2/7 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (3/9 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• purine nucleobases degradation II (anaerobic) (12/24 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• gallate degradation III (anaerobic) (2/11 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (3/17 steps found)
• superpathway of methanogenesis (2/21 steps found)
• superpathway of L-lysine degradation (6/43 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• One carbon pool by folate
• Propanoate metabolism
• Purine metabolism
• Pyruvate metabolism
• Taurine and hypotaurine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.2, 2.7.2.1

Use Curated BLAST to search for 2.1.2.- or 2.1.2.2 or 2.7.2.1 or 2.7.2.15 or 6.3.1.21

Protein Sequence (393 amino acids)

>HMPREF1078_RS10980 formate-dependent phosphoribosylglycinamide formyltransferase (Parabacteroides merdae CL09T00C40)
MVTIGTPKSATATKVLLCGSGELGKEFVIELQRYGVEVIALDKYADAPAMQVAHRSYVVS
MLDGDALREIVEKEKPDYIVPEVEAIATQTLMELEKEGFHVIPTARATWLTMNREGIRRL
AAEELGLPTSPYRFAETEEEFIEAVKVIGMPCVVKPIMSSSGHGQSIIRKEEDIDRSWHY
AQEGGRAGAGKVIVEGFVDFDYEITQLTVRHIGGTSFLEPVGHVQVDGDYRESWQPQAMN
PVAKEKAREIAGKITEALGGRGIFGVELFVKGEDVIFSEVSPRPHDTGMVTMITQDLSQF
ALHARAILGLPIPNIAFHGAGASRAVVVEGDSNQLSLGNLDKVLEQPDTQMRFFGKPEVH
GHRRMAVLLARGLDVADARRKTGVMMEQLEIKL