Protein Info for HMPREF1058_RS15835 in Phocaeicola vulgatus CL09T03C04

Annotation: radical SAM protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 356 PF04055: Radical_SAM" amino acids 14 to 152 (139 residues), 51.2 bits, see alignment E=1.7e-17 PF13186: SPASM" amino acids 238 to 300 (63 residues), 39.4 bits, see alignment E=6e-14

Best Hits

KEGG orthology group: None (inferred from 62% identity to dol:Dole_1807)

Predicted SEED Role

"Radical SAM domain heme biosynthesis protein" in subsystem Heme biosynthesis orphans

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9A3K5 at UniProt or InterPro

Protein Sequence (356 amino acids)

>HMPREF1058_RS15835 radical SAM protein (Phocaeicola vulgatus CL09T03C04)
MVNKCSLHPTDASIILTYRCPMKCKMCNIWFNPTNKSEEIKASDLKSLPKLKFINLTGGE
PFIREDLPEIVEECYKHTDRIVISTSGWFEDRVIALAKQFPIIGIRISIEGLSCKNDELR
GHAGGFDKGLRTLLALKEMGLKDIGFGCTVSNNNSKDMLSLYQLSKSLGMEFATAAFHNS
YYFHKDDNVITNKNEVCGDFEQLIEWQLKENHPKSWFRAWFNMGLINYIEGGRRMLPCEA
GSANFFIDPFGDVFPCNGLEEKYWKKSMGNIHETPDFMTIWTSKKAEEVRAMVRKCPKNC
WMVGTASPVMHKYIKYPLKWALQNKLRSMQGKTVCLDKKWCDVGQDPCQGDLREKF