Protein Info for HMPREF1058_RS06630 in Phocaeicola vulgatus CL09T03C04

Annotation: glucose-1-phosphate thymidylyltransferase RfbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01207: glucose-1-phosphate thymidylyltransferase
  • amino acids 2 to 289 (288 residues), 485.6 bits, E=2.1e-150
• PF00483: NTP_transferase
  • amino acids 2 to 240 (239 residues), 254.5 bits, E=1.1e-79
• PF12804: NTP_transf_3
  • amino acids 3 to 128 (126 residues), 34.8 bits, E=1.9e-12

Best Hits

Swiss-Prot: 67% identical to RMLA_NEIMB: Glucose-1-phosphate thymidylyltransferase (rmlA1) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K00973, glucose-1-phosphate thymidylyltransferase [EC: 2.7.7.24] (inferred from 99% identity to bvu:BVU_3949)

MetaCyc: 66% identical to glucose-1-phosphate thymidylyltransferase (Escherichia coli O52)
Glucose-1-phosphate thymidylyltransferase. [EC: 2.7.7.24]

Predicted SEED Role

"Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 2.7.7.24)

MetaCyc Pathways

• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• O-antigen building blocks biosynthesis (E. coli) (9/11 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
• dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
• dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
• dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-α-D-fucose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
• dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
• dTDP-β-L-olivose biosynthesis (3/7 steps found)
• dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (2/6 steps found)
• dTDP-D-desosamine biosynthesis (2/6 steps found)
• dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
• dTDP-β-L-megosamine biosynthesis (3/8 steps found)
• dTDP-β-L-mycarose biosynthesis (2/7 steps found)
• dTDP-α-D-forosamine biosynthesis (3/9 steps found)
• dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of erythromycin biosynthesis (2/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (11/33 steps found)
• superpathway of megalomicin A biosynthesis (3/22 steps found)
• superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.24

Sequence Analysis Tools

See I9U531 at UniProt or InterPro

Protein Sequence (295 amino acids)

>HMPREF1058_RS06630 glucose-1-phosphate thymidylyltransferase RfbA (Phocaeicola vulgatus CL09T03C04)
MKGIVLAGGSGTRLYPITKGVSKQLLPVFDKPMIYYPISVLMLAGIREILIISTPYDLPS
FKRLLGDGSDFGVRFEYAEQPSPDGLAQAFIIGEKFIGNDSVCLVLGDNIFHGNGLSAML
KESVRAAEEDKKATVFGYWVSDPERYGVAEFDKEGNCLSIEEKPAQPKSNYAVVGLYFYP
NKVVEVAKNIKPSARGELEITTVNQQFLQDKELKVQTLGRGFAWLDTGTHDSLAEASTYI
EVIEKRQGLKVACLEGIALRKGWITPEKMQELAQPMLKNQYGQYLLKVIKELGLE