Protein Info for HMPREF1058_RS05000 in Phocaeicola vulgatus CL09T03C04

Annotation: DUF4010 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13194: DUF4010
  • amino acids 188 to 398 (211 residues), 171.5 bits, E=1.1e-54

Best Hits

KEGG orthology group: None (inferred from 100% identity to bvu:BVU_3811)

Predicted SEED Role

"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Sequence Analysis Tools

See I9IPV1 at UniProt or InterPro

Protein Sequence (429 amino acids)

>HMPREF1058_RS05000 DUF4010 domain-containing protein (Phocaeicola vulgatus CL09T03C04)
MEKLYDYLPQQLVTFILVTLFSLLIGLSQRRISLKREGETTLFGTDRTFTFIGILGYLLY
ILDPKEMHLFMGGGIILGMLLGINYYVKQSQFHVFGVTTIIIALITYCMAPIISTQPSWF
YVMMVVTVLLFTEMKHTFTELVQRMQNDEMITLAKFLAISGIILPMLPKEPLIPGINLTP
YTIWLATVVVSGISYLSYLLKQYVFRESGTLVSGIIGGLYSSTATISILARKCKRIQAHE
IPEYVSAMMLAVSMMFLRFMILILIFSTSIFLNIYPYLLIMSATAAGIGIFIHKKHTIKG
EREVIEETETESNPLEFKVALIFALLFVVFTFLTHYTLVYAGTGGLSILSIATGLSDITP
FILNLLQGGNIPATIVGACIMQAIISNIVVNMFYAIFFSSRRKEMLPYVWKGFLTVIGVN
AVLLLIYYF