Protein Info for H281DRAFT_02471 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 687 PF00724: Oxidored_FMN" amino acids 5 to 344 (340 residues), 216.3 bits, see alignment E=3.1e-67 PF07992: Pyr_redox_2" amino acids 391 to 639 (249 residues), 53 bits, see alignment E=1.5e-17 PF01134: GIDA" amino acids 391 to 422 (32 residues), 21.5 bits, see alignment (E = 4.5e-08) PF00070: Pyr_redox" amino acids 391 to 425 (35 residues), 23.4 bits, see alignment (E = 2.8e-08) PF12831: FAD_oxidored" amino acids 392 to 429 (38 residues), 32.7 bits, see alignment 2.1e-11 PF00890: FAD_binding_2" amino acids 392 to 431 (40 residues), 26.4 bits, see alignment 1.6e-09 PF13450: NAD_binding_8" amino acids 394 to 428 (35 residues), 32.4 bits, see alignment (E = 3.8e-11)

Best Hits

KEGG orthology group: None (inferred from 98% identity to bug:BC1001_5840)

MetaCyc: 62% identical to N,N-dimethylglycine/sarcosine dehydrogenase (ferredoxin) (Chromohalobacter salexigens)
RXN-22743 [EC: 1.5.7.3]; 1.5.7.3 [EC: 1.5.7.3]

Predicted SEED Role

"DgcA Dimethylglycine demethylase subunit A"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.7.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MHD7 at UniProt or InterPro

Protein Sequence (687 amino acids)

>H281DRAFT_02471 2,4-dienoyl-CoA reductase (Paraburkholderia bryophila 376MFSha3.1)
MRYPNLFKPLTLNKLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAVCGG
SSPVSIDSPQGWWKSVNLSTDKIIDPLSRLAEAMHAHGAKIMIQATHMGRRSAFHGEHWP
HLMSPSGVREPVHRGNAKIIEVEEIRRIITDFAAAAKRVKDAGMDGVEISAAHQHLIDQF
WSPRTNFRTDEWGGSLENRLRFGVEVLQAVREAVGPDFCVGLRMCGDEFHEDGLDHEQLK
EIAQAMSESGLIDYLGVIGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVVKVPVMHA
QSIRDAGQAERLLASGMVDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
GLDVLCVQNAATSREATMPHVIARTNGPKRKVVVVGAGPAGLEAARVAKSRGHDVVLFEK
NDYVGGQIMLAAKAPQREQMAGIVRWFDMETKRLGVDRRLGVAADDKTIMAEKPDIVVLA
TGGSAFTSQVAAWGVDEGLAVSSWDVLSGKVEPGKNVLVYDGVSTHAGAGVADFISSRGS
NVEIVTPDVKVADDVGGTTFPIFYRRLYAQGVVHTPNYWLDKVYEEDGKKIAVIRNEYTE
EQEERAVDQVVIENGSTPNDELYWKLKPESVNRGQVDVHKLFASEPQPSLSEELGNGRFL
LFRVGDCISMHNIHGAIYDALRLCKDF