Protein Info for H281DRAFT_01513 in Paraburkholderia bryophila 376MFSha3.1

Annotation: hippurate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 TIGR01891: amidohydrolase" amino acids 20 to 382 (363 residues), 371.8 bits, see alignment E=2e-115 PF01546: Peptidase_M20" amino acids 79 to 391 (313 residues), 160.7 bits, see alignment E=4.8e-51 PF07687: M20_dimer" amino acids 194 to 290 (97 residues), 42.7 bits, see alignment E=4.7e-15

Best Hits

Swiss-Prot: 41% identical to ILL8_ORYSJ: IAA-amino acid hydrolase ILR1-like 8 (ILL8) from Oryza sativa subsp. japonica

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 94% identity to bug:BC1001_5267)

MetaCyc: 46% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MJY5 at UniProt or InterPro

Protein Sequence (396 amino acids)

>H281DRAFT_01513 hippurate hydrolase (Paraburkholderia bryophila 376MFSha3.1)
MSESARYTEVADLIPAAESLREIRHHIHHHPELAYEELQTGALVAGKLEEWGWQVTRGVG
QTGVVGTLKVGDGKRSIGIRADMDALPIIEQTGLPYASGTHGKMHACGHDGHTTILLGAA
QRLAATRNFSGTVHLYFQPAEESGIDSGAKKMIEDGLFERFPCDAVFGLHNHPGAEPGVL
LFRKGPFMSAGDKAIITVEGVGGHAARPHLTVDPVVVAASIVMALQTIVARNVDPSQPAV
VTVGSMHAGTANNVIASTARLELSVRSFSPEVRALLKKRITELAQTQAASYGGKAVVEYI
EGYPVVVNSDDETDFAVEVARELVGNDRVVAQTDILMGSEDFAFMLQKRPGTFLRIGNGV
GEDGCMVHNPHYDFNDQNLPVGAAFWTRLVERYLSR