Protein Info for Ga0059261_3964 in Sphingomonas koreensis DSMZ 15582

Updated annotation (from data): Imidazolonepropionase (EC 3.5.2.7)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (IMIDAZOLONEPROPIONASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: imidazolonepropionase (EC 3.5.2.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01224: imidazolonepropionase
  • amino acids 26 to 395 (370 residues), 459.1 bits, E=4.9e-142
• PF01979: Amidohydro_1
  • amino acids 59 to 373 (315 residues), 67.7 bits, E=1.1e-22
• PF07969: Amidohydro_3
  • amino acids 109 to 375 (267 residues), 62.3 bits, E=6.5e-21

Best Hits

Swiss-Prot: 73% identical to HUTI_SPHWW: Imidazolonepropionase (hutI) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01468, imidazolonepropionase [EC: 3.5.2.7] (inferred from 73% identity to swi:Swit_4633)

MetaCyc: 56% identical to imidazolonepropionase (Pseudomonas fluorescens)
Imidazolonepropionase. [EC: 3.5.2.7]

Predicted SEED Role

"Imidazolonepropionase (EC 3.5.2.7)" in subsystem Histidine Degradation (EC 3.5.2.7)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.7

Sequence Analysis Tools

See A0A2M8WI78 at UniProt or InterPro

Protein Sequence (395 amino acids)

>Ga0059261_3964 Imidazolonepropionase (EC 3.5.2.7) (Sphingomonas koreensis DSMZ 15582)
MAIPADTLWRNARLATMTGDGLGIVEQGAIAATGGTISYIGPDSDAPDAAEIVDCQGRWI
TPGLVDCHTHLVHAGNRAREFEARLEGATYEEIARAGGGILSTVTATRAASEDELIASAL
PRLDQLIAEGVTTVEIKSGYGLTLDDELKMLRAARSLGEARNVHIRTTLLAAHAVPPEYK
GDADGYVDLVCETIIPAAIGHADAVDAFCEGIGFSPEQTDRVLAAAVAHGLPVKLHAEQL
SNLHGAALAAHHNALSADHLEHLDEAGIAAMAASGTVATLLPGAFYFTREEHRPPIAALR
AAGVPIALATDCNPGTSPMASILLAANMGATFFRLTVTECLRGITVNGARALGLRTGTLE
PGGPADLAIWNVTDLAELVYRIGANPLHSRIYAGT