Protein Info for Ga0059261_2298 in Sphingomonas koreensis DSMZ 15582

Updated annotation (from data): prephenate and/or arogenate dehydrogenase (EC 1.3.1.13)
Rationale: Important for fitness in defined media. 31% identical to arogenate dehydrogenase from Arabidopsis (Q9LMR3). The substrate could be prephenate (dehydrogenation first) or arogenate (transamination followed by dehydrogenation).
Original annotation: Prephenate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07991: IlvN
  • amino acids 2 to 82 (81 residues), 21.2 bits, E=4.8e-08
• PF03807: F420_oxidored
  • amino acids 3 to 87 (85 residues), 32.5 bits, E=2.7e-11
• PF03446: NAD_binding_2
  • amino acids 4 to 101 (98 residues), 24.3 bits, E=7.2e-09
• PF02153: PDH_N
  • amino acids 17 to 133 (117 residues), 68.3 bits, E=1.4e-22

Best Hits

KEGG orthology group: K00210, prephenate dehydrogenase [EC: 1.3.1.12] (inferred from 64% identity to pzu:PHZ_c2752)

Predicted SEED Role

"Prephenate and/or arogenate dehydrogenase (unknown specificity) (EC 1.3.1.12)(EC 1.3.1.43)" in subsystem Chorismate Synthesis or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• L-tyrosine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis III (2/4 steps found)
• 4-hydroxy-3-prenylbenzoate biosynthesis (1/4 steps found)
• superpathway of chorismate metabolism (37/59 steps found)
• superpathway of novobiocin biosynthesis (2/19 steps found)

KEGG Metabolic Maps

• Novobiocin biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.13 or 1.3.1.43

Sequence Analysis Tools

See A0A1L6J750 at UniProt or InterPro

Protein Sequence (249 amino acids)

>Ga0059261_2298 prephenate and/or arogenate dehydrogenase (EC 1.3.1.13) (Sphingomonas koreensis DSMZ 15582)
MKRFGIIGFGRFGQLAARHLRDHFSVVVTDTADVGEAAAAIGVKTGSLADAADCDVVMLA
VPVQAMAATIAAIAPLVRPGALVLDVASVKMLPARWMLEALPESVDIVATHPLFGPQSAR
GGLEGQPLVVCAVRGERHHKVAEFGRSLGLSVSITTAEEHDREMAYVQALTHLIGRALVN
IRIPDEELKTNSYQHLLELCGLIRDDSKELFFAIQNLNPYAEEITRQFIAEANGLLAESL
KAGNRSAAD