Protein Info for Ga0059261_1896 in Sphingomonas koreensis DSMZ 15582
Updated annotation (from data): Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26)
Rationale: Specifically important for: D-Xylose. The KEGG annotation is vague; xylose is oxidized to alpha-ketoglutarate semialdehyde (SEED_correct)
Original annotation: NAD-dependent aldehyde dehydrogenases
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to KGSD2_AZOBR: Alpha-ketoglutaric semialdehyde dehydrogenase 2 from Azospirillum brasilense
KEGG orthology group: K14519, NADP-dependent aldehyde dehydrogenase [EC: 1.2.1.4] (inferred from 62% identity to sjp:SJA_C1-05970)MetaCyc: 58% identical to alpha-ketoglutaric semialdehyde dehydrogenase subunit (Pseudomonas putida KT2442)
2,5-dioxovalerate dehydrogenase. [EC: 1.2.1.26]
Predicted SEED Role
"Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Proline, 4-hydroxyproline uptake and utilization (EC 1.2.1.26)
MetaCyc Pathways
- D-xylose degradation V (5/5 steps found)
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- D-xylose degradation III (4/5 steps found)
- cytosolic NADPH production (yeast) (4/5 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- pyruvate fermentation to acetate VIII (1/2 steps found)
- D-galactarate degradation II (1/3 steps found)
- D-glucarate degradation II (1/3 steps found)
- L-carnitine degradation II (1/3 steps found)
- L-lyxonate degradation (1/3 steps found)
- D-arabinose degradation III (3/6 steps found)
- D-glucuronate degradation II (2/5 steps found)
- 1,2-dichloroethane degradation (1/4 steps found)
- trans-4-hydroxy-L-proline degradation II (1/4 steps found)
- L-arabinose degradation III (2/6 steps found)
- D-galacturonate degradation II (1/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (12/31 steps found)
- superpathway of pentose and pentitol degradation (12/42 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.26 or 1.2.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2M8WCE8 at UniProt or InterPro
Protein Sequence (524 amino acids)
>Ga0059261_1896 Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (Sphingomonas koreensis DSMZ 15582) MELTGGLFIGGERRQSDARFHAYDPAAGADIADAGFASASEQDVADACAAAEAAFLPYST KPLEARARFLETIADEIEALGDVLIERACRESGLPAARITGERGRTVGQLRLFAKEVRDG AWQKLRIDHADRERTPPRPDLRLRMVPLGPVAVFGASNFPLAFSTAGGDTASAFAAGCPV VVKGHRAHPGTAELIATAIIRAVETCGMPAGTFGMVNGTSRKVGETLVADPRIQAVGFTG SRGGGEALMRIAAARPRPIPVYAEMAAINPVILMPQALKARGPALAEAFVASLAMGAGQF CTNPGLVMGIDGPELDAFVARAGEVLSGQAAQVMLTDGIWEAFESGKAKLAGSAFVTKVA EGVEADGPNRGRAALFSVAGKDFLADPVHLHEVFGVSSVVVRCASLEELKAVLGELEGQL TATLQVDEGDYPEAQALLPVLERTVGRVIANGWPTGVEVTHAMVHGGPYPSTSDPRSTSV GTLAIDRFLRPVSYQDLPEALLPAALRENAQAGTVARIDGSWTI