Protein Info for Ga0059261_1778 in Sphingomonas koreensis DSMZ 15582

Updated annotation (from data): beta-fructosidase for D-raffinose catabolism (EC 3.2.1.26)
Rationale: Specifically important for: D-Raffinose pentahydrate. Uncertain if it acts on raffinose or sucrose (raffinose could be cleaved to galactose + sucrose first). Alternatively, SEED suggests that it hydrolyzes sucrose-6-phosphate, but the basis for this is unclear.
Original annotation: Beta-fructosidases (levanase/invertase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00251: Glyco_hydro_32N
  • amino acids 30 to 342 (313 residues), 292.2 bits, E=6.1e-91
• PF08244: Glyco_hydro_32C
  • amino acids 345 to 494 (150 residues), 104.1 bits, E=7.8e-34

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

• sucrose degradation III (sucrose invertase) (4/4 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• inulin degradation (1/3 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.B3

Sequence Analysis Tools

See A0A2M8WC72 at UniProt or InterPro

Protein Sequence (502 amino acids)

>Ga0059261_1778 beta-fructosidase for D-raffinose catabolism (EC 3.2.1.26) (Sphingomonas koreensis DSMZ 15582)
MKKLAILLTAAAVLASAASAQQADVRPGYHYGPARNWMNDPNGLVYYDGEYHLFYQYNPH
GDRWGHMNWGHAVSRDLVNWEELPVAIPETDVMAFSGTAVIDWNNTTGFGKNGKPPMIAI
YTGHDPKTERQSQYLAYSNDRGRTFTIHGEVLNAGNEKHFRDPKVFWHAPTRRWVMVVLK
AAANTAEIYTSPNLKDWTHRSSFGPAGGRGKFWECPDLFELPVEGGAPGETRWVLSINLG
DNAIGGGSGGQYFVGDFDGEKFTLVPGWPAAPQWMDYGADFYATISWNDMPKGDPRRVWM
GWANDWRYAEAIPTWPARGIMTVARTVALRKTAEGYHLLQAPVRELATLRGTPQRAPALA
LSETPVPLPIEGGKADIELELDTGTADQVSIALTDGQGWQTRIGVNPTVNEVFVDRTRSG
PHFHDGFANRHVAPVDLKSRKVKLRVLADESIVEVFVNDGRQTITDRFYRGGGALTWSAT
ARGGKATMNLTAWPMRSMESRK