Protein Info for PGA1_c09980 in Phaeobacter inhibens DSM 17395

Annotation: kynureninase KynU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01814: kynureninase
  • amino acids 10 to 380 (371 residues), 293.8 bits, E=9.7e-92
• PF00266: Aminotran_5
  • amino acids 99 to 334 (236 residues), 65.7 bits, E=3.9e-22
• PF22580: KYNU_C
  • amino acids 289 to 376 (88 residues), 118.2 bits, E=1.3e-38

Best Hits

Swiss-Prot: 49% identical to KYNU_PSEFL: Kynureninase (kynU) from Pseudomonas fluorescens

KEGG orthology group: K01556, kynureninase [EC: 3.7.1.3] (inferred from 80% identity to sil:SPO2824)

MetaCyc: 49% identical to kynureninase subunit (Pseudomonas fluorescens)
Kynureninase. [EC: 3.7.1.3]

Predicted SEED Role

"Kynureninase (EC 3.7.1.3)" in subsystem NAD and NADP cofactor biosynthesis global (EC 3.7.1.3)

MetaCyc Pathways

• L-tryptophan degradation I (via anthranilate) (2/3 steps found)
• NAD de novo biosynthesis II (from tryptophan) (6/9 steps found)
• 3-hydroxy-4-methyl-anthranilate biosynthesis II (2/5 steps found)
• L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde (2/5 steps found)
• superpathway of NAD biosynthesis in eukaryotes (8/14 steps found)
• L-tryptophan degradation IX (3/12 steps found)
• L-tryptophan degradation XII (Geobacillus) (3/12 steps found)
• L-tryptophan degradation III (eukaryotic) (5/15 steps found)
• L-tryptophan degradation XI (mammalian, via kynurenine) (6/23 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (14/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (10/42 steps found)

KEGG Metabolic Maps

• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.3

Sequence Analysis Tools

See I7DNR4 at UniProt or InterPro

Protein Sequence (397 amino acids)

>PGA1_c09980 kynureninase KynU (Phaeobacter inhibens DSM 17395)
MTDFSATKAMFSLPEGMIYLDGNSLGPMPRATAERVRGTIEDEWGEMLITGWNKAGWMQK
PTAIGDRIARLIGAEPGHVVMGDTLSIKVYQAVASALELNPTRKVVLSDNGNFPSDLYMV
EGLVKSLGPDYSLRVVAPEEVSANLTDDIAVLMLTEVDYRTGRKHDMKALTEQAHAAGVV
TVWDLAHSAGALPVDLAGCKADFAVGCTYKYLNSGPGGPAFIYVAPRHAEKARPALSGWL
GHAAPFDFDLNYKPGNGIERMRVGTPPVLQLAALEASMDIWDMADMADVRAKSIELCDLF
ITEVESRCPDLTLASPRDGTVRGSQVSFRFHEGYAAMQALIARGVVGDFRSPDIMRFGFT
PLYIDQGDVRAAVDIIEDVITNALWDSAEYKTRNAVT