Protein Info for Psest_0097 in Pseudomonas stutzeri RCH2

Annotation: phosphonates metabolism transcriptional regulator PhnF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 242 TIGR02325: phosphonate metabolism transcriptional regulator PhnF" amino acids 18 to 240 (223 residues), 171.6 bits, see alignment E=1e-54 PF00392: GntR" amino acids 19 to 79 (61 residues), 48.3 bits, see alignment E=6.2e-17 PF07702: UTRA" amino acids 101 to 237 (137 residues), 95.2 bits, see alignment E=3.2e-31

Best Hits

KEGG orthology group: K02043, GntR family transcriptional regulator, phosphonate transport system regulatory protein (inferred from 71% identity to pmy:Pmen_2887)

Predicted SEED Role

"Transcriptional regulator PhnF" in subsystem Alkylphosphonate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH06 at UniProt or InterPro

Protein Sequence (242 amino acids)

>Psest_0097 phosphonates metabolism transcriptional regulator PhnF (Pseudomonas stutzeri RCH2)
MRLSKKTMQLSRQPSEPLYLELAQVLRGELRHYRIGDFLPGEMQLARRFAVNRHTLRRAV
DELINEGRLLRRQGKGTQVLQRPLVYPLQADSAYSDALAALGVRTEARLLERRQLSASAE
EARHLQLAEGAALIELSTLRLLDGEPVSLIRHRYCASHAELLADYQGGSLRRYLGERGLP
LTRRFSLIGARLADRDEAAQLLMPLRMPLLSVFTLSCDASGRPLEIALSASRSDRFQYQV
AT