Protein Info for PGA1_c09770 in Phaeobacter inhibens DSM 17395

Annotation: aerobic cobaltochelatase subunit CobT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 626 PF06213: CobT" amino acids 8 to 280 (273 residues), 336.6 bits, see alignment E=1.3e-104 TIGR01651: cobaltochelatase, CobT subunit" amino acids 11 to 611 (601 residues), 807.1 bits, see alignment E=5.8e-247 PF11775: CobT_C" amino acids 393 to 609 (217 residues), 334.4 bits, see alignment E=3.3e-104

Best Hits

Swiss-Prot: 54% identical to COBT_SINSX: Aerobic cobaltochelatase subunit CobT (cobT) from Sinorhizobium sp.

KEGG orthology group: K09883, cobaltochelatase CobT [EC: 6.6.1.2] (inferred from 88% identity to sit:TM1040_0642)

MetaCyc: 54% identical to CobT (Pseudomonas denitrificans (nom. rej.))

Predicted SEED Role

"Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DNN8 at UniProt or InterPro

Protein Sequence (626 amino acids)

>PGA1_c09770 aerobic cobaltochelatase subunit CobT (Phaeobacter inhibens DSM 17395)
MAKKPNDNPADPFKKALAEATKVMANDPELNVSYSVDPAGMSGDSMRLPQVSRRMSREEV
LLARGTADALALRHKFHDTATHARYAPPGDMARDLYEALESARCEAMGARHMPGTASNID
VKIQNEALRKGYDQIKSATEAPLAVSAGYLIRHLATGRPMPEAAANVMELWRGFIEQQAG
ETLANLDDTIEDQATFAKFARQVITDLGYGDQLGEDPDQLDDEQENDAEEDAEEQQDPDS
NGQDDSDEEEADANPEQAQEQQQDESQAQVSMDEMADDEFAEDTEMPDGEAPLDPPPPPA
VSEADPDYKVFNDAHDEEIAAEDLAEPAELERLRAYLDQQLEPLKGAVSRLANKLQRRLQ
AQQNRSWEFDLEEGILDAGRLARVVANPTTPLSFKREKDTEFRDTVVTLLLDNSGSMRGR
PISIAAICADVLARTLERCNVKVEILGFTTRAWKGGLAREAWLNDGRPQLPGRLNDLRHI
IYKGADAPMRRTRDNLGLMMKEGLLKENIDGEALEWAHRRMIARQEARKILMVISDGAPV
DDSTLSVNPANYLEKHLRDVIAMVERRKQVELLAIGIGHDVTRYYDRAVTITDVEQLAGA
MTEQLAALFDSDPRARARVMGIKRAS