Protein Info for PGA1_c09650 in Phaeobacter inhibens DSM 17395
Annotation: UDP-N-acetylenolpyruvoylglucosamine reductase MurB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to MURB_RUEPO: UDP-N-acetylenolpyruvoylglucosamine reductase (murB) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
KEGG orthology group: K00075, UDP-N-acetylmuramate dehydrogenase [EC: 1.1.1.158] (inferred from 80% identity to sil:SPO1200)Predicted SEED Role
"UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)" in subsystem Peptidoglycan Biosynthesis or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 1.1.1.158)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
- peptidoglycan recycling I (8/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.158
Use Curated BLAST to search for 1.1.1.158
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7DYZ6 at UniProt or InterPro
Protein Sequence (307 amino acids)
>PGA1_c09650 UDP-N-acetylenolpyruvoylglucosamine reductase MurB (Phaeobacter inhibens DSM 17395) MNIDGLRGKLHPNRDLSGLTWLQVGGPADYLFQPVDIEDLSHMLRSLDPSVPVFPMGVGS NLIVRDGGLRALVIRLGRGFNTIEIADDTVVAGAAALDGHVARKAADAGIDLTFLRTIPG SIGGAVRMNAGCYGSYMADVFQSATVVLRSGEVVTLRGDDLKFAYRQSDLPEGAVLVSAV LRGPKGDPEALHARMEEQLAKRDATQPTKDRSAGSTFRNPAGFSSTGQADDVHDLKAWKV IDDAGMRGARRGAAQMSEKHSNFLINTGGASAADLEGLGEDVRKKVYANSGITLEWEIMR VGDPLSK