Protein Info for Psest_0968 in Pseudomonas stutzeri RCH2

Annotation: carbamoyl-phosphate synthase, large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1053 (1052 residues), 1572.4 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 7 to 125 (119 residues), 163.1 bits, E=8e-52
  • amino acids 558 to 669 (112 residues), 165.2 bits, E=1.8e-52
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 240.7 bits, E=4.2e-75
  • amino acids 673 to 875 (203 residues), 117.3 bits, E=2.5e-37
• PF02787: CPSase_L_D3
  • amino acids 427 to 505 (79 residues), 89.2 bits, 4.9e-29
• PF02655: ATP-grasp_3
  • amino acids 678 to 845 (168 residues), 27.9 bits, E=7.9e-10
• PF02222: ATP-grasp
  • amino acids 679 to 845 (167 residues), 45.2 bits, E=3.1e-15
• PF07478: Dala_Dala_lig_C
  • amino acids 693 to 843 (151 residues), 27 bits, E=1e-09
• PF02142: MGS
  • amino acids 958 to 1041 (84 residues), 77.9 bits, 1.8e-25

Best Hits

Swiss-Prot: 67% identical to CARB_BUCAI: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 77% identity to abo:ABO_0318)

MetaCyc: 76% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See L0GHT3 at UniProt or InterPro

Protein Sequence (1073 amino acids)

>Psest_0968 carbamoyl-phosphate synthase, large subunit (Pseudomonas stutzeri RCH2)
MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALKEEGFRVILVNSNPATIMTDPSM
ADATYIEPIKWATVAKIIEKERPDALLPTMGGQTALNCALDLEKHGVLAKFGVEMIGANA
DTIDKAEDRSRFDKAMKDIGLACPVSGIAHNMEEAYGVLDKVGFPCIIRPSFTMGGTGGG
IAYNREEFEEICARGLDLSPTSELLIDESLIGWKEYEMEVVRDKKDNCIIVCSIENFDPM
GVHTGDSITVAPAQTLTDKEYQILRNASLAVLREIGVETGGSNVQFGICPNTGRMVVIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELSNDITGGRTPASFEPAIDYVVTKIP
RFAFEKFPKADARLTTQMKSVGEVMAIGRTFQESMQKALRGLEVGATGFDPKLNLADAEA
ESTLKRELTVPGADRIWFVADAFRAGKTVAEVFELTRIDEWFLVQIEDLVRDEEHVKTLG
LSSIDRDLMYKLKRKGFSDARLAKLLGVTEKNLRSHRHKLKVLPVYKRVDTCAAEFATDT
AYMYSTYEEECEANPSSREKIMILGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNC
NPETVSTDYDTSDRLYFEPVTLEDVLEIVRVEQPKGVIVQYGGQTPLKICRALEEAGVPI
IGTSPDAIDRAEDRERFQQMVQRLNLRQPQNATARSEEEAIAASKAIGYPLVVRPSYVLG
GRAMEIVYEEEELKRYMREAVQVSNDSPVLLDHFLNCAIEVDIDAVCDGTDVVIGGIMQH
IEQAGVHSGDSACSLPAYSLPQHIQDEIRDQVRKMALELDVIGLMNVQMAVQGEDIYVLE
VNPRASRTVPFVSKCIGESLAKVAARVMAGKTLKEIGFTREIIPTYYSVKEAVFPFAKFP
GVDTILGPEMKSTGEVMGVGDSFAEAFAKAQLGASEILPTSGCAFLSVREDDKPYVEQVA
RDLVGLGFEVVATAGTARIIEAAGLPVRRVNKVTEGRPHVVDMIKNDEVTLVINTTEGRQ
SIADSYSIRRNALQHKICITTTIAGGQAICEALKFGPEKTVRRLQDLHAGIKA