Protein Info for PGA1_c09450 in Phaeobacter inhibens DSM 17395

Annotation: DNA helicase II UvrD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 787 PF00580: UvrD-helicase" amino acids 31 to 295 (265 residues), 310.7 bits, see alignment E=2.8e-96 PF13245: AAA_19" amino acids 35 to 278 (244 residues), 75 bits, see alignment E=1.6e-24 PF13361: UvrD_C" amino acids 300 to 466 (167 residues), 116.5 bits, see alignment E=4.6e-37 amino acids 530 to 636 (107 residues), 97.8 bits, see alignment E=2.2e-31

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 75% identity to rsq:Rsph17025_0678)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DYX3 at UniProt or InterPro

Protein Sequence (787 amino acids)

>PGA1_c09450 DNA helicase II UvrD (Phaeobacter inhibens DSM 17395)
MSSFDDIDPYEGISLSARAMAARPMPYLDGLNPAQRAAVECLEGPVLMLAGAGTGKTKAL
TARIVHLLTTGTAKPNEILAVTFTNKAAREMKERVAGMLGQAVEGMPWLGTFHSICVKLL
RRHAELVGLKSNFTILDADDQIRLLKQLIKAEGIDDKRWPARMLAGVIDDWKNRALTPSK
VPVSDASAYNHRGVDFYAQYQARLKDLNAVDFGDLLLHMVTIFQDNPDVLEQYQRWFRFI
MVDEYQDTNVAQYLWLRLLAGAHKNICCVGDDDQSIYGWRGAEVGNILRFEKDFPGANVV
RLEQNYRSTEHILSAASGVIRGNSGRLGKELWTEARGGEKVRLIGHWDGEEEARWIGEEI
EAMQRGTRGLAPVDLNNMAILVRASHQMRAFEDRFLTIGLPYRVIGGPRFYERLEIRDAM
AYFRQVISPDDDLAFERIVNTPKRGLGDKAQQTIQTMARSNGVSLVEGARLAVESGQIKG
KGGKALHQLVTDLARWGRLAGDADTTHMELAEIILDESGYTTMWQADKNPDSPGRLENLK
ELVKALEAFENLQGFLEHVSLIMDNDKQDAAQKVSIMTLHAAKGLEFPAVFLPGWEDGLF
PSQRSMDESGLKGLEEERRLAYVGITRAEEICTISFAGNRRVFGQWQSQLPSRFIDELPE
ENVEVLTPPGLYGGGYGAAAPSGGGGAVRSTIEDRMAAADGYNSPGWKRMQSRAGERGLS
QPRESKSSVIDLTATSAFMVGERVFHQKFGYGAITGIEGDKLEVEFEKAGTKKVVARFVT
AAEDVPF