Protein Info for Psest_0091 in Pseudomonas stutzeri RCH2

Annotation: phosphonate C-P lyase system protein PhnL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 TIGR02324: phosphonate C-P lyase system protein PhnL" amino acids 4 to 226 (223 residues), 354.9 bits, see alignment E=7.9e-111 PF00005: ABC_tran" amino acids 27 to 181 (155 residues), 103.4 bits, see alignment E=7.7e-34

Best Hits

Swiss-Prot: 63% identical to PHNL_ECOLI: Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL (phnL) from Escherichia coli (strain K12)

KEGG orthology group: K05780, putative phosphonate transport system ATP-binding protein (inferred from 86% identity to pae:PA3375)

MetaCyc: 63% identical to ATP-binding cassette protein PhnL (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Phosphonates transport ATP-binding protein PhnL" in subsystem Alkylphosphonate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHA8 at UniProt or InterPro

Protein Sequence (234 amino acids)

>Psest_0091 phosphonate C-P lyase system protein PhnL (Pseudomonas stutzeri RCH2)
MNTLIEVRDLAKTFTLHQQHGVSLQVLRGLSFTVAAGECLVLHGQSGAGKSTLLRTLYGN
YLPAGGSIRIRHAGDWLELVGAEPRQVLAVRRQTLGYVSQFLRVIPRVPALEVVMEPALA
RGWTRADAEARAKALLTRLNIPERLWQLAPGTFSGGEQQRVNLARGFMVSWPVMLLDEPT
ASLDEANARVVLELIDEAKAAGSALIGIFHDRAIREAVADRYLDMTQEDLAHAS