Protein Info for PGA1_c09110 in Phaeobacter inhibens DSM 17395

Annotation: thiamine biosynthesis protein ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 13 to 84 (72 residues), 98.1 bits, E=2.1e-32
• PF01964: ThiC_Rad_SAM
  • amino acids 124 to 566 (443 residues), 625 bits, E=5.9e-192
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 124 to 569 (446 residues), 702.9 bits, E=6.6e-216

Best Hits

Swiss-Prot: 92% identical to THIC_RUEST: Phosphomethylpyrimidine synthase (thiC) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 92% identity to sit:TM1040_2054)

MetaCyc: 76% identical to HMP-P synthase monomer (Caulobacter vibrioides)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See I7DYU0 at UniProt or InterPro

Protein Sequence (599 amino acids)

>PGA1_c09110 thiamine biosynthesis protein ThiC (Phaeobacter inhibens DSM 17395)
MNVPNPKITTGALPASRKIYVSGEVHPDIRVPMREISTHPTAGEAPLPVYDSSGPYTDPD
VLTDIRQGLPQLRRQWIEARGDVESYQGRDVKPEDNGFVEDDRLTPEFPVKPAPLRGKDG
TAPTQLAYARAGIITPEMEYVAIRENQLRELSPCHRDGNDFGANIPDYVTPEFVRDEIAA
GRAIIPANINHPEIEPMIIGRNFLVKINANMGTSAVTSSMEEEVDKLVWAIRWGADTVMD
LSTGRNIHNTREWIVRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLIEQAEQGVDY
FTIHAGVRLHMVPMTVERVTGIVSRGGSIMAKWCLHHHKESFLYEHFEDICDICRQYDVS
FSLGDGLRPGSIADANDEAQFAELETLGELTKIAWAKDCQVMIEGPGHVAMHKIKENMDK
QLECCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPDRDD
VKTGVITYKIAAHAADLAKGLPGAQRRDDALSRARFEFRWEDQFNLSLDPDTAREFHDQT
LPKQAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGMEAMAAKFREGGELYVPAPETAE