Protein Info for Psest_0088 in Pseudomonas stutzeri RCH2

Annotation: phosphonate metabolism protein PhnP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR03307: phosphonate metabolism protein PhnP" amino acids 1 to 249 (249 residues), 386.4 bits, see alignment E=2.6e-120 PF12706: Lactamase_B_2" amino acids 67 to 221 (155 residues), 69.6 bits, see alignment E=1.4e-23

Best Hits

Swiss-Prot: 51% identical to PHNP_ECOLI: Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase (phnP) from Escherichia coli (strain K12)

KEGG orthology group: K06167, PhnP protein (inferred from 73% identity to pst:PSPTO_2567)

MetaCyc: 51% identical to 5-phospho-alpha-D-ribosyl 1,2-cyclic phosphate phosphodiesterase (Escherichia coli K-12 substr. MG1655)
RXN0-6710 [EC: 3.1.4.55]

Predicted SEED Role

"Metal-dependent hydrolases of the beta-lactamase superfamily I; PhnP protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFA4 at UniProt or InterPro

Protein Sequence (249 amino acids)

>Psest_0088 phosphonate metabolism protein PhnP (Pseudomonas stutzeri RCH2)
MRLTLLGTGGAAQVPVYNCSCPACRAAQLDPNRRRRACSALVECGEQRWLLDSGLPDLCE
RFAPGSFSGILQTHYHADHAQGLLQLRWGTGLRIPVHGPDDPDGLADLYKHPGILDFSEP
FAAFEQRMLGTLRVTALPLVHSKPTFGYLLEGHGRRTAYLTDTVGLPDSTRQLLQDTALD
VLVLDCSTPPRDKPPRNHNDLTRALQSIEELQPAQAVLTHIGHELDAWFMQAPRELPGNV
RLAHDGMTL