Protein Info for Psest_0876 in Pseudomonas stutzeri RCH2

Annotation: Type III restriction enzyme, res subunit./Type I restriction enzyme R protein N terminus (HSDR_N).

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1064 PF04313: HSDR_N" amino acids 108 to 221 (114 residues), 38.5 bits, see alignment E=2.9e-13 PF04851: ResIII" amino acids 294 to 454 (161 residues), 50.1 bits, see alignment E=7.5e-17 PF18766: SWI2_SNF2" amino acids 297 to 553 (257 residues), 79.8 bits, see alignment E=5.9e-26 PF00270: DEAD" amino acids 304 to 500 (197 residues), 34 bits, see alignment E=6.3e-12 PF22679: T1R_D3-like" amino acids 674 to 727 (54 residues), 37.3 bits, see alignment 9.6e-13

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 93% identity to psa:PST_0639)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFH1 at UniProt or InterPro

Protein Sequence (1064 amino acids)

>Psest_0876 Type III restriction enzyme, res subunit./Type I restriction enzyme R protein N terminus (HSDR_N). (Pseudomonas stutzeri RCH2)
MADSKEAQFQQDIITAMVAQGWLTGPASGYDRRTALYTEDFLGYFKDAWPERWDKFAKAN
PNNPDDVLVQKLVRELEQSGTLDVLRHGFKLPAVKVELCSFQPDHGMNPDTLKRYWCNRL
RVVPEVSYSPHAREAGSSGQSYNPRLDLVLFVNGIPTATLELKSEFKQSVENAKRQYRYD
RPVKDPLTRKPEPLLTFKRGALVHFAVGQNEVAMTTKLDGKDTFFLPFNLGSAEGGAGNP
PPPDDSQYATGYLWQQLFQPDAWLKVLGRFLHLDKKTSEGFDGSLTTRETMIFPRYHQWD
VVNQLIQTTHAEGPGKRYLIQHSAGSGKSNSIAWTAHQLASMYSDDGQRLFSSVIVVTDR
TVLDSQLQNTIYQFVHAQGVVRPITRDVGNQSKSEQLAEALAEQTRIIIVTIQTFPALFD
ALEKYPKLASGRYAVIADEAHSSQTGSSASKLKQILGSDALDAEEISAEDLLDAAVQARQ
PNERISYYAFTATPKAKTLELFGRVPDPTLPPSASNRPEAFHLYSMRQAIEEGFILDVLQ
NYTTYSTAWKIAHPDGEDDEVDSKKARMKLARWVRLHPYNIDKKVEIIVEHFRGNIRHLL
GGQAKAMVVTSSRQEAVRYQLAVKAYVQQMGYADVHPLVAFSGSVLPDEVIPEEVTESSS
LLNAGLNGRDLADAFDTQDFNVMIAANKYQTGFDQPKLCAMYVDKKLQGVDCVQTLSRLN
RTFPAKQTFILDFFNEPQDILDAFLPYYTKAELSDVTDPQIIYDLQKKLDAEGIYHWQEV
EAFALAFFDPKAAASKLSYYCQPAKERFAKRYAFSVESRQQALDYKRTAEANGDSAGLKK
AEHALKEAGEQVDQLDLFRKNLQSFVRLYEFLSQIVPYEDRELEQLCVFAKHLHPLLRVD
RLQQDDVDVGELQLTHYRLSKRAEHQLRLSEEGGEYTLKPGSDVGSGKPHDPEKKRLSEI
IDALNDIFGAEVSDEDQLHFLTGIAERISRQEDVMAQVNNHSVDQVMHGLLPKRVIDTVL
DAMTDQEKLSLEVLDNETKSRAFALVIYKMLKALAGGAHPSAGL