Protein Info for Psest_0864 in Pseudomonas stutzeri RCH2

Updated annotation (from data): maltose ABC transporter, permease component 1 (MalF)
Rationale: Specifically important for utilizing D-Maltose monohydrate
Original annotation: ABC-type sugar transport systems, permease components

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 521 transmembrane" amino acids 30 to 48 (19 residues), see Phobius details amino acids 54 to 71 (18 residues), see Phobius details amino acids 80 to 104 (25 residues), see Phobius details amino acids 289 to 314 (26 residues), see Phobius details amino acids 324 to 348 (25 residues), see Phobius details amino acids 379 to 401 (23 residues), see Phobius details amino acids 435 to 456 (22 residues), see Phobius details amino acids 492 to 514 (23 residues), see Phobius details PF20872: MalF_N_TM" amino acids 28 to 74 (47 residues), 36.8 bits, see alignment 4e-13 PF14785: MalF_P2" amino acids 113 to 269 (157 residues), 136.9 bits, see alignment E=1e-43 PF00528: BPD_transp_1" amino acids 308 to 515 (208 residues), 62.1 bits, see alignment E=8.8e-21

Best Hits

KEGG orthology group: K10109, maltose/maltodextrin transport system permease protein (inferred from 94% identity to psa:PST_3485)

Predicted SEED Role

"Maltose/maltodextrin ABC transporter, permease protein MalF" in subsystem Maltose and Maltodextrin Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GI50 at UniProt or InterPro

Protein Sequence (521 amino acids)

>Psest_0864 maltose ABC transporter, permease component 1 (MalF) (Pseudomonas stutzeri RCH2)
MNARAELPSPAMTRPTSWGLPRFLTTGVRWLLWLAFNALSLYLVVALYVQKQMAFALLGL
VVTGIASYLFINRRMYAQRYIFPSVAGMLVFVIFPLLYTVGIGFTNYSGTNLLSQAQVER
YHLSQTYLAGERFRFTLHQSPDGERLRVDKGELGVFVSPPLTGEPDPEAPLSLLPAESVE
GLGEALALREVIQRRKVLEQWVMQAPDGSLLRLYGLREVAAVEPQYRQDGPGVLVETRTG
ARLTADMERGFYVDETGKAVPPGFTVFTGFANFSRVLTEPSIREPFMQIFAWTFAFAGLT
VVFTLAVGLVLASLLQWELVRGKAFYRLMLILPYAVPGFISILVFRGLFNQNFGEINLLL
EGLFGIRPDWFSDPSLARTMILIVNTWLGYPYMLLLCMGLLQAIPRDQYEASAIDGASPL
DNLLRITLPQLIKPLMPLLIACFAFNFNNFVLITLLTRGGPDIIGATTPAGTTDLLVSYT
YRIAFQDSGQDFALAAAIATMIFILVGAMALLNLKLSKVKV