Protein Info for Psest_0840 in Pseudomonas stutzeri RCH2

Annotation: anaerobic ribonucleoside-triphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF03477: ATP-cone" amino acids 14 to 97 (84 residues), 65.4 bits, see alignment E=5.8e-22 TIGR02487: anaerobic ribonucleoside-triphosphate reductase" amino acids 126 to 658 (533 residues), 458.9 bits, see alignment E=1.3e-141 PF13597: NRDD" amino acids 127 to 657 (531 residues), 602.7 bits, see alignment E=7.5e-185

Best Hits

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 96% identity to psa:PST_3509)

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFA5 at UniProt or InterPro

Protein Sequence (672 amino acids)

>Psest_0840 anaerobic ribonucleoside-triphosphate reductase (Pseudomonas stutzeri RCH2)
MPRQAEVAKPVSLRKRDGRLAAFEVDKIARAIAAAGAATGEFEAAVAHALAEAVRLPLAH
RTAVEVEQVQDGVERALMAAGYFDTARAYIVYRERHARLRRDRKAVVDVATSMNEYLSRE
DWRVRANANQGYSLGGLILNVSGKVTANYWLDEVYSPAIGTAHREGDLHIHDLDMLAGYC
AGWSLRTLLNEGFNGIPGRVEAGPPKHLSSALGQMVNFLGTLQNEWAGAQAFSSFDTYLA
PFVRKDRLGFDEVRQAIQEFIYNLNVPSRWGTQTPFTNLTFDWVCPEDLREQIPYIGGEE
MPFAYGELQAEMELINRAYIEVMQAGDGLGRVFTFPIPTYNITHDFPWDSENAERLFEMT
ARYGLPYFQNFLNSDMQPNQVRSMCCRLQLDVRELLKRGGGLFGSAEQTGSLGVVTVNCA
RLGYLYRGDKAALLEQLDVLLDMARQSLEVKRKVIQHHMDAGLYPYTKRYLGTLRNHFST
IGVNGLHEMVRNFTDDTEGLQTAAGRALAIEVLDHVRARLVQFQEDTGHLYNLEATPAEG
TTYRFAKEDRKRFPDILQAGSAEAPYYTNSSQLPVGFTDDPFEALELQDELQCKYTGGTV
LHLYMAEQISSAEACKKLVRNALSRFRLPYLTVTPTFSICPVHGYLAGEHEFCPKCDEAL
LHKQQAEAALAV