Protein Info for PGA1_c08340 in Phaeobacter inhibens DSM 17395

Annotation: methionyl-tRNA synthetase MetG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 PF00133: tRNA-synt_1" amino acids 4 to 246 (243 residues), 27.5 bits, see alignment E=2.1e-10 TIGR00398: methionine--tRNA ligase" amino acids 7 to 558 (552 residues), 461.1 bits, see alignment E=2.7e-142 PF09334: tRNA-synt_1g" amino acids 7 to 412 (406 residues), 477.3 bits, see alignment E=4.8e-147 PF19303: Anticodon_3" amino acids 424 to 575 (152 residues), 254.6 bits, see alignment E=4.4e-80

Best Hits

Swiss-Prot: 89% identical to SYM_RUEST: Methionine--tRNA ligase (metG) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 89% identity to sit:TM1040_2197)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DN79 at UniProt or InterPro

Protein Sequence (575 amino acids)

>PGA1_c08340 methionyl-tRNA synthetase MetG (Phaeobacter inhibens DSM 17395)
MDDMARFLITSAIPYINGIKHLGNLVGSQLPADLYARYQRARGHEVMFLCATDEHGTPAE
LAAAKAGKPVADYCAEMHEVQAGIAKGFGLSFDHFGRSSSPQNHALTQHFAGKLAEQGLI
REVTEQQVYSHTDGRFLPDRYIEGTCPNCGYERARGDQCEECTKQLDPTDLIDPRSAISG
STDLEVRETKHLYLCQSQLKDQLDDWINSKSDWPVLTTSIAKKWLHDGDGLQDRGITRDL
DWGVPVKKGDADWPGMEGKVFYVWFDAPIEYIAASREWADANGKTDADWQRWWRTDMGAE
DVKYVQFMGKDNVPFHTLSFPATILGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGVFM
DQALEILPADYWRWWLLSHAPESSDSEFTWENFQQSVNKDLADVLGNFVSRITKFCRSKY
GEAVPAGNDYGEAEQALIDEISTRVTAYEGHMAAMDVRKSAQELRAIWVAGNEYLQSVAP
WSVFKTDPDQAAAQVRLGLNLIRLYAVLSAPFIPDATERLLAALQTEDRSWPDDVAAALS
ALPAGHAFTVPEVLFAKITDEQREEWQERFSGTRD