Protein Info for Psest_0816 in Pseudomonas stutzeri RCH2

Annotation: periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 201 transmembrane" amino acids 25 to 45 (21 residues), see Phobius details TIGR02161: periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family" amino acids 14 to 194 (181 residues), 325.6 bits, see alignment E=4.6e-102 PF03264: Cytochrom_NNT" amino acids 21 to 193 (173 residues), 259.8 bits, see alignment E=1.7e-81 PF22113: Mtrc-MtrF_II-IV_dom" amino acids 56 to 190 (135 residues), 32.9 bits, see alignment E=7.1e-12

Best Hits

Swiss-Prot: 98% identical to NIRT_PSEST: Denitrification system component NirT (nirT) from Pseudomonas stutzeri

KEGG orthology group: K02569, cytochrome c-type protein NapC (inferred from 96% identity to psa:PST_3531)

MetaCyc: 53% identical to NapC (Aliivibrio fischeri)
RXN-15816 [EC: 7.1.1.8]

Predicted SEED Role

"Cytochrome c-type protein NapC" in subsystem Nitrate and nitrite ammonification or trimethylamine N-oxide (TMAO) reductase

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.1.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJB1 at UniProt or InterPro

Protein Sequence (201 amino acids)

>Psest_0816 periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family (Pseudomonas stutzeri RCH2)
MTDQDGNKQKKGGILALLRRPSTRYSLGGILVVGIVAGIIFWGGFNTALEATNTETFCIS
CHEMGDNVYPEYKETIHYANRTGVRATCPDCHVPRDWTHKMVRKVEASKELWGKIVGTID
TAEKFEAKRLTLARREWSRMRASDSRECRNCHSLESMSSDMQKQRARKQHEMAREDNLTC
IACHKGIAHHLPEGMTEEDED