Protein Info for PGA1_c07840 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): Beta-glucosidase (EC 3.2.1.21)
Rationale: Specifically important for utilizing D-Cellobiose. Automated validation from mutant phenotype: the predicted function (3.2.1.21) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: beta-glucosidase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00232: Glyco_hydro_1
  • amino acids 7 to 433 (427 residues), 444.3 bits, E=2.2e-137
• TIGR03356: beta-galactosidase
  • amino acids 9 to 434 (426 residues), 565.8 bits, E=3.1e-174

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 79% identity to sit:TM1040_3309)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

• neolinustatin bioactivation (2/3 steps found)
• linamarin degradation (1/2 steps found)
• lotaustralin degradation (1/2 steps found)
• linustatin bioactivation (2/4 steps found)
• cellulose degradation II (fungi) (1/3 steps found)
• coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
• α-tomatine degradation (1/6 steps found)
• firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Phenylpropanoid biosynthesis
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

See I7EUV9 at UniProt or InterPro

Protein Sequence (444 amino acids)

>PGA1_c07840 Beta-glucosidase (EC 3.2.1.21) (Phaeobacter inhibens DSM 17395)
MFRNSRADFPKDFLFGTATSAYQIEGHGFGGAGPTHWDSFAATPGNVVRAEHGQRACDHY
HRYAEDLDLAAAAGFDCYRFSTSWARVMPEGRGAPNPEGLDFYDRLTDAILERGLKPCVT
LYHWELPQALADLGGWRNAEIANWFGDYAEVIMGRIGDRMYSAAPINEPWCVGWLSHFLG
HHAPGLRDIRATARAMHHVMLAHGTAIQAMRALGMSNLGGVFNLEWATPVDDSEAAQQAA
ARYDAIYNGFFLGGAFHGRYPDLALEGLEPHLPKGWQDDFATITAPVDWCGLNYYTRKQI
APDAGPWPQYAEVDGPLPKTQMGWEIYPQGLYDFLTRTARDYTGDLPLIVTENGMANADV
VTKGKVEDAARITFVDDHLDAVRRAIADGVPVQGYFLWSLLDNYEWALGYEKRFGLVHVD
FETLKRTPKASYHALRSALTGASK