Protein Info for PGA1_c07390 in Phaeobacter inhibens DSM 17395

Annotation: alpha-galactosidase RafA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 PF16875: Glyco_hydro_36N" amino acids 30 to 247 (218 residues), 112.4 bits, see alignment E=5.5e-36 PF02065: Melibiase" amino acids 260 to 586 (327 residues), 364.8 bits, see alignment E=8.8e-113 PF16874: Glyco_hydro_36C" amino acids 609 to 686 (78 residues), 39 bits, see alignment E=1.9e-13

Best Hits

KEGG orthology group: K07407, alpha-galactosidase [EC: 3.2.1.22] (inferred from 68% identity to rde:RD1_2865)

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EUS0 at UniProt or InterPro

Protein Sequence (699 amino acids)

>PGA1_c07390 alpha-galactosidase RafA (Phaeobacter inhibens DSM 17395)
MSTVRDIRCWTLHDQRQSLILAAHGTDLAEVIYWGPRLPDGTDLEMLVAATRMDVTGGML
DAVPPLSICPEARRSFPGQPGLSLTNSAGEALLPRFTFERAEEDDQTLTLLYHDAKNGLR
YRARFELTLATGMIRATASLTSEQPVSVNWLSAPVFPAPQNSHEMIDFHGRWIGEFQTSR
TPWSVGARLRDNPTGRTGHEHFPGLIVPCTGATNSQGSAYAFHYGWSGGHKMLAEELPDG
RRQIQWGHATGSRRSPGTQFETAPLYAVFSDQGINGCAIAFQRHLRDEILQPPTPARPRP
VHYNCWEAVYFDHDHATLCDIASRAAALGAERFVLDDGWFGRRDDDTTSLGDWTIDRRKY
PDGLMPLINHVHAEGMTFGIWFEPEMINSDSDLFRAHPDWVLGDPDQVLGRQQMVLNIAL
PEVQAYLYERISAVLADHPIDYVKWDHNRVLPEPDSAQAEAIYVLFDRLRADFPTVEFES
CSSGGGRIDFGILSRTHRVWLSDSNDPLERLRIQHEAALFLPMVVTGSHVGPRKCHTSGR
VTDIRLRAWVAAQRHMGFEMDPRELTEEEADVLRQVTRWWKGNRHWTERADILRLDSDDP
AVIAELQRAEDGSRFVAFAGLSAASAQILPRPLRLTGLDPGTLYEISLLNRDDAPGLSRG
SPLLKTETARLSGQYLMHQGITLPWTFPQSIWVIEGTRL