Protein Info for Psest_0734 in Pseudomonas stutzeri RCH2

Annotation: RND family efflux transporter, MFP subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 53 to 354 (302 residues), 187 bits, see alignment E=2.2e-59 PF16576: HlyD_D23" amino acids 67 to 222 (156 residues), 46.2 bits, see alignment E=7.2e-16 PF13533: Biotin_lipoyl_2" amino acids 68 to 114 (47 residues), 40.5 bits, see alignment 3.5e-14 PF13437: HlyD_3" amino acids 163 to 266 (104 residues), 34.5 bits, see alignment E=6e-12

Best Hits

KEGG orthology group: None (inferred from 92% identity to psa:PST_3617)

Predicted SEED Role

"FIG00963798: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHR9 at UniProt or InterPro

Protein Sequence (359 amino acids)

>Psest_0734 RND family efflux transporter, MFP subunit (Pseudomonas stutzeri RCH2)
MTSTPAFPVSKAWLTIIACALPLLGGCSSEAEPAPEPPRVALVEPLRPAESKAEALRFSG
VVQSVTSTQLSFEVSGRIERILIDEGTRVRRGQTLAQLDRTDYRLQLREAEARLRQLEAD
LARKRTLLAEGILAPAAIEALQANTVAARVARDSAQRNIDHSTLTAPFDGVVARRLAEPD
MVVAVGTPVFEMQDNRHIEVSVDLPESAALSIPLGPELQAEAELVIADLRLPLRYKEHST
QPREGARTYRLVLQGEPPEDFNLLPGMAMRVRLERPAQPETDNDGFRLPLSALQTGSDGK
HFIWQAADGRARRLAVELQQVEGDQALIRGDALQAQLPIIVAGGSKLHDDQPIEARERN