Protein Info for Psest_0071 in Pseudomonas stutzeri RCH2

Annotation: UDP-galactopyranose mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 38% identical to FCF2_ECOLX: dTDP-fucopyranose mutase (fcf2) from Escherichia coli

KEGG orthology group: K01854, UDP-galactopyranose mutase [EC: 5.4.99.9] (inferred from 95% identity to psa:PST_2890)

MetaCyc: 38% identical to dTDP-fucopyranose mutase (Escherichia coli O52)
RXN-14533 [EC: 5.4.99.59]

Predicted SEED Role

"UDP-galactopyranose mutase (EC 5.4.99.9)" in subsystem LOS core oligosaccharide biosynthesis or linker unit-arabinogalactan synthesis (EC 5.4.99.9)

MetaCyc Pathways

O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
UDP-α-D-galactofuranose biosynthesis (2/2 steps found)
dTDP-β-D-fucofuranose biosynthesis (3/4 steps found)
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (7/19 steps found)
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (14/33 steps found)
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (2/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.59 or 5.4.99.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH88 at UniProt or InterPro

Protein Sequence (406 amino acids)

>Psest_0071 UDP-galactopyranose mutase (Pseudomonas stutzeri RCH2)
MRDMSPQDRNPERLGGGAGEEPQARRHGFDYLIVGAGFAGSVLAERLARGLNKRVLVVDR
RPHIAGNAYDYHDESGVLIHRYGPHIFHTNAQRIVDYLSQFTEWRPYEHRVLAQVDGQEV
PIPINMTTLNRLYGLNMTTEEEAADFLASRAEPVENIQTSEDVVINGIGRELYQKFFRGY
TRKQWGLDPSQLDKSVTSRIPTRTNTDDRYFTDSFQQMPKHGYTRMFEKMLAHPNIKVML
NTDYREIRDEVQYDHLIFCGPIDEYFDYRFGKLPYRSLKFEHKQLEQEQFQAVGTVNYPS
EDVPYTRISEYKHLTGQVHPKTSVTYEYPAAEGDPYYPIPRPENAELYKRYQQLADQTPG
VTFVGRLGTYKYYNMDQVVGQALALYKRIEEAELATQPQAVPEQLG