Protein Info for Psest_0721 in Pseudomonas stutzeri RCH2

Annotation: tryptophanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 TIGR00233: tryptophan--tRNA ligase" amino acids 1 to 333 (333 residues), 320.8 bits, see alignment E=5.6e-100 PF00579: tRNA-synt_1b" amino acids 3 to 281 (279 residues), 175.8 bits, see alignment E=6.6e-56

Best Hits

Swiss-Prot: 77% identical to SYW_PSESM: Tryptophan--tRNA ligase (trpS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 97% identity to psa:PST_3630)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.2

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ15 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Psest_0721 tryptophanyl-tRNA synthetase (Pseudomonas stutzeri RCH2)
MTTRILTGITTTGTPHLGNYAGAIRPAVLASRDAAADSFYFLADYHALIKCDDPLRIQRS
RLEIAATWLACGLDVERVTFYRQSDVPEIPELTWLLTCVTAKGLLNRAHAYKASVDKNVE
SGEDPDAGVTMGLYSYPVLMAADILMFNAHKVPVGRDQIQHVEMARDIAQRFNHLFGNGR
EFFTLPEAVIEEGVATLPGLDGRKMSKSYDNTIPLFGSAKELKAAVARIVTDSRLPGEPK
DPDNSHLFTLYQAFATAQQQADFRRELLGGLAWGDAKQRLFELLDSELGEARELYHQLIT
KPAELEDILQAGAAKARRIATPFLGELREAVGLRNFRSEVKSAAPAKKKSSKVARFASFR
EADGAFRFRFFAADGEELLLSRPLSNPKAIGSLTQRLIAGGPDALELREDEGDQFTLWLD
DECIADSPHFESAEALDAAMLRVREALATLVE