Protein Info for Psest_0007 in Pseudomonas stutzeri RCH2

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 PF11563: Protoglobin" amino acids 23 to 161 (139 residues), 45.5 bits, see alignment E=1.3e-15 PF21118: DosC_2nd" amino acids 182 to 287 (106 residues), 78.1 bits, see alignment E=9e-26 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 293 to 453 (161 residues), 165.2 bits, see alignment E=5.3e-53 PF00990: GGDEF" amino acids 295 to 450 (156 residues), 153.6 bits, see alignment E=5.9e-49

Best Hits

KEGG orthology group: K13069, diguanylate cyclase [EC: 2.7.7.65] (inferred from 92% identity to psa:PST_0006)

Predicted SEED Role

"Putative Heme-regulated two-component response regulator" in subsystem Putative hemin transporter or cAMP signaling in bacteria

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGS2 at UniProt or InterPro

Protein Sequence (456 amino acids)

>Psest_0007 diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MQQIPNHQLVTEWLRLLDGYPAEAAAALNAVAEEKSAELAEHFYSQMLADPHSASFLSHD
QVKNRLGKSMQKWIVTVCSSTAHSDLDAVVDLQRKVGEIHARIEIPVSLVLRGARSLKGR
LRELLEQRDLGEPVRRKAARMGADLIDLAVEIICFAYSQSHDRNSRAEEAYRLFSVSQNI
GVEKERQRAALLDWENQLMFDLAIGNLSGALPQLAGSEFGLWFRHKASHAFQGSPESASI
LDYIERIDAELKAIATLQETGERLAKLHHIREQTRSIKFLLDSLFEQANDLEAGRDVLTR
LLNRKFLPVVMAKEVLYARQSNNAFGVLAIDVDHFKRINDTYGHEAGDQVLQQVGALLAS
NTRGGDYAFRLGGEEFLLVLVDIDASKALAAAEKLRERIAREHFRLSSGDVLQVTVSVGM
AVHDGHPDYERLLQQADRALYQAKNGGRNRCVLHQD