Protein Info for Psest_0709 in Pseudomonas stutzeri RCH2

Annotation: pseudouridine synthase, RluA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 transmembrane" amino acids 224 to 243 (20 residues), see Phobius details TIGR00005: pseudouridine synthase, RluA family" amino acids 72 to 365 (294 residues), 318.5 bits, see alignment E=2.2e-99 PF01479: S4" amino acids 73 to 118 (46 residues), 28.3 bits, see alignment 1.2e-10 PF00849: PseudoU_synth_2" amino acids 147 to 294 (148 residues), 112.1 bits, see alignment E=2.9e-36

Best Hits

Swiss-Prot: 85% identical to RLUD_PSEAE: Ribosomal large subunit pseudouridine synthase D (rluD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06180, ribosomal large subunit pseudouridine synthase D [EC: 5.4.99.12] (inferred from 98% identity to psa:PST_3642)

MetaCyc: 56% identical to 23S rRNA pseudouridine1911/1915/1917 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11837 [EC: 5.4.99.23]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIR7 at UniProt or InterPro

Protein Sequence (375 amino acids)

>Psest_0709 pseudouridine synthase, RluA family (Pseudomonas stutzeri RCH2)
MMSAPVRRDKDAVFKHKRVAETKGRSAICGPRVRPLPADVTCLSQTNLSRRMSTTSKQAI
QLHAEVPFDLGGQRLDQVAAQLFSEHSRSRLAAWIKDGLLTVDGAVLRPRDTVHAGAVLE
LNAEQQAQGEWVAQDIPLEIVYEDDQILVIDKPAGLVVHPAAGHADGTLLNALLHHVPGL
INVPRAGIVHRLDKDTTGLMVVAKTLQAQTRLVEQLQARSVSRIYEAIVIGVIITGGTID
APIGRHGQQRQRMAVVEGGKPAVSHYRVLERFRSHTHVRVKLETGRTHQIRVHMPHLGYP
LVGDPLYAGRFRIPPAANPTLVQTLREFPRQALHARFLELDHPVTGQRMKWESPLPDDFV
WLLTLLQQDREAFVG