Protein Info for PGA1_c07030 in Phaeobacter inhibens DSM 17395

Annotation: phenylalanyl-tRNA synthetase beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 797 (797 residues), 659.4 bits, see alignment E=4.2e-202 PF01588: tRNA_bind" amino acids 46 to 140 (95 residues), 67.1 bits, see alignment E=3e-22 PF03483: B3_4" amino acids 219 to 390 (172 residues), 227.1 bits, see alignment E=2.7e-71 PF03484: B5" amino acids 410 to 467 (58 residues), 57.5 bits, see alignment 3.2e-19 PF17759: tRNA_synthFbeta" amino acids 490 to 683 (194 residues), 167.9 bits, see alignment E=5.9e-53 PF03147: FDX-ACB" amino acids 704 to 797 (94 residues), 76.4 bits, see alignment E=4.7e-25

Best Hits

Swiss-Prot: 82% identical to SYFB_RUEPO: Phenylalanine--tRNA ligase beta subunit (pheT) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 82% identity to sit:TM1040_3623)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DMT5 at UniProt or InterPro

Protein Sequence (798 amino acids)

>PGA1_c07030 phenylalanyl-tRNA synthetase beta chain (Phaeobacter inhibens DSM 17395)
MKFTLSWLKDHLETDASVEDITETLTDLGLEVEGVSNPADRLKDFTLGYVKHAEKHPDAD
KLRVCKVDTDEGEMQIICGAPNAREGITVVVCKPGMYIPGLDITIGVGKIRGVESFGMMA
SERELELSDEHDGIIELPSGEVGDRFVDWLAQNAPAKVDPVIEIAITPNRPDALGVRGIA
RDLAARGLGTLKPLAIEPVEGGYDSPLKVDIAEDTLDGCPLFTGRLIRGVKNGPSPQWLQ
DRLSAIGLRPISTLVDITNYFTFDQNRPLHVFDAAKVAGNLRVHRAVGGETLTALDEKDY
TLSEGQMVISDDNGAESIAGIMGGVATGCTEETTDVFLESAFWDHVQIATTGRALKINSD
ARYRFERGVDPEYTRDGLDHATRMILDLCGGEPSNVVTAGAVPEHARAYKLDPERVQSLV
GMDIPESEQRQTLTRLGFRLEGNMAHVPSWRPDVMGEADLVEEVARIASLTKLEGKPLPR
LTDGVPAPVMTPQQRRQQIARRTCASLGYNEVVSYTFIDKASAALFGGGDDATMLANPIS
SEMSHMRPALLPGLLQAAARNQARGFMDLALFEAGPAFHGGEPGEQHNLISGLLVGRTGP
KDVHGASRAVDTFDAKADIEAVLAAIGAPAKVQILRGAQSWWHPGRHGKICLGPKKVLGV
FGELHPKVLSEMGIKGSAVAFTIWPDEVPMPRKSGANRGALVQSDLQAVERDFAFVVADD
VEALTLVNAAVGADKALIDDVRVFDEFIGGSLGEGSKSLAITVRLQPTDKTLTEKDIEAV
SAKIVAKVTKATGGTLRG