Protein Info for Psest_0700 in Pseudomonas stutzeri RCH2

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 185 to 206 (22 residues), see Phobius details amino acids 213 to 235 (23 residues), see Phobius details amino acids 251 to 269 (19 residues), see Phobius details amino acids 281 to 298 (18 residues), see Phobius details amino acids 304 to 323 (20 residues), see Phobius details amino acids 334 to 353 (20 residues), see Phobius details amino acids 364 to 385 (22 residues), see Phobius details PF07696: 7TMR-DISMED2" amino acids 36 to 172 (137 residues), 98.2 bits, see alignment E=6.1e-32 PF07695: 7TMR-DISM_7TM" amino acids 183 to 387 (205 residues), 134.9 bits, see alignment E=5.5e-43 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 445 to 607 (163 residues), 161.2 bits, see alignment E=8.9e-52 PF00990: GGDEF" amino acids 449 to 605 (157 residues), 170.2 bits, see alignment E=4.8e-54

Best Hits

KEGG orthology group: None (inferred from 67% identity to pfv:Psefu_4092)

Predicted SEED Role

"FIG00954617: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH05 at UniProt or InterPro

Protein Sequence (620 amino acids)

>Psest_0700 diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MHAVLLVLLLCLGTLLPAHADTALIGAHDSGRNLNAYVELLMDPGAELEVEDLQRPEVQA
RFQPAQGKASVGQSPHPWWVKVTLQRASEAPAQWWLEVGSVTLKDLRLYLPDGDGGWHGR
QSGELVGFHEGRDHAYRRMLFRLPELSNQRPLTFYLRSYDPAGNSFPLKVWQLDALQEQA
VGENLFLGLIYGLILAMLLYNLFIFLSLRDTAYFWYVMTTSGALLMILGMTGHGFQYLWP
NGPVPFWLDRISIPALWGFSACRFTQTLLQTRRFVPWAHRLLTFALCLYVTAVLLNIFGL
RAFGAWVFVALALTAIPASLWASFRRWRQGYFPALLYLCGFGVILGSVNLLLLRATGVIQ
PAAWSGYVFPLAVAAESILFSFALASRIQILKQERAAALEHADREKTERLKHMQASAEHL
SLAVEARTAELAQANRDLSEREIELKQAAFHDPLTELPNRRYLIERIEAAMAEAARRDES
IALLLIDLDHFKPINDDHGHDAGDLLLRTLGQRLRGLVRSSDMAARLGGDEFAILLTGAN
VEQDASQVAERLLYELSLPVPYRGNSLAVTVSVGAAFFPHHADEFTQLYKAADEALYRAK
QQGRASWVQQDGCLPVDQPI