Protein Info for Psest_0669 in Pseudomonas stutzeri RCH2

Annotation: Ethanolamine ammonia-lyase, large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 PF06751: EutB" amino acids 12 to 443 (432 residues), 735.5 bits, see alignment E=1e-225

Best Hits

Swiss-Prot: 53% identical to EUTB_ECO57: Ethanolamine ammonia-lyase heavy chain (eutB) from Escherichia coli O157:H7

KEGG orthology group: K03735, ethanolamine ammonia-lyase large subunit [EC: 4.3.1.7] (inferred from 96% identity to psa:PST_3681)

MetaCyc: 53% identical to ethanolamine ammonia-lyase subunit alpha (Escherichia coli K-12 substr. MG1655)
Ethanolamine ammonia-lyase. [EC: 4.3.1.7]

Predicted SEED Role

"Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7)" in subsystem Ethanolamine utilization (EC 4.3.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.7

Use Curated BLAST to search for 4.3.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHL2 at UniProt or InterPro

Protein Sequence (463 amino acids)

>Psest_0669 Ethanolamine ammonia-lyase, large subunit (Pseudomonas stutzeri RCH2)
MAYSHSVGGTTWRFDDLREVMAKASPARSGDLLAGVAADSDAERVAAQMCLAAVPLKRFL
EEALIPYESDEVTRLIIDSHDDAAFAPVSHLTVGDFRNWLLGDDANESSLKALAPGLTPE
MVAAVSKIMRVQDLILVAQKVRVVTRFRNTIGLAGRMSTRLQPNHPTDHGAGIAASILDG
LLYGNGDAVIGINPATDSTSGICELLKMLDAVIGRYEIPTQACILTHVTTSIEAINRGAP
LDLVFQSIAGTEAANASFGISLATLQEGYEAGLSQNRGTLGDNLMYFETGQGSALSANAH
HGVDQQTCEARAYAVARSYKPLLVNTVVGFIGPEYLYNGKQIIRAGLEDHFCGKLLGVPM
GCDICYTNHAEADQDDMDMLLTLLGTAGINFIMGIPGSDDVMLNYQTTSFHDALYVRQVL
GLRPAPEFEEWLAKMQILRQDGNRLQLGRELPEAFRRALEKMA