Protein Info for Psest_0667 in Pseudomonas stutzeri RCH2

Annotation: Phosphodiesterase/alkaline phosphatase D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF16655: PhoD_N" amino acids 43 to 131 (89 residues), 67.6 bits, see alignment E=1.2e-22 PF09423: PhoD" amino acids 144 to 497 (354 residues), 343.6 bits, see alignment E=1.3e-106

Best Hits

KEGG orthology group: K01113, alkaline phosphatase D [EC: 3.1.3.1] (inferred from 92% identity to psa:PST_3683)

Predicted SEED Role

"Phosphodiesterase/alkaline phosphatase D"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIM8 at UniProt or InterPro

Protein Sequence (517 amino acids)

>Psest_0667 Phosphodiesterase/alkaline phosphatase D (Pseudomonas stutzeri RCH2)
MSAPDQKRRQLLQGIGAGLALPALGVAPAIIAAPRERPQMLDGVQSGDVAGDRALIWSRC
DRPGRLIVEWDTRSRFGNPRRMVSSITDAGQDFTARVDLRGLPADQSIFYRARFEDARSG
MLSEPWFGHLRSAPTRPRDIRFVWGGDTCGQGYGINPDFGGMRIYESMRMRRPDFFLHSG
DVIYADGPIPAEVTAEDGSIWRNLVTEEKSKVAETLNEFRGNYRYNLLDENLRAFNAEVP
QIWQWDDHEVTNNWSPSKQLDDRYQQVRDIDTLVQRARQAYLEYAPMRMARGDTQGRIYR
KVSYGPLLDVFVLDMRSYRGPNTHNLQTEQGADTVFLGREQLRWLKRELRASRATWKVIA
ADMPIGLHVPDGPRWEAIANGHDGEALGRELEIAELLTFIQRARVRNTVWLTADVHYCAA
HYYHPNRAAYQQFEPFWEFVAGPLNAGSFGPNPLDGTFGPEVVFQKAPPTANSSPRAGFQ
FFGEVEIDAQTAALQVSLRDIDGTVVYSQTLEPWQQA