Protein Info for Psest_0651 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 797 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 171 to 192 (22 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 251 to 366 (116 residues), 53.7 bits, see alignment E=2.3e-18 PF13188: PAS_8" amino acids 255 to 296 (42 residues), 34.7 bits, see alignment 3.6e-12 PF00989: PAS" amino acids 255 to 356 (102 residues), 47.7 bits, see alignment E=4.5e-16 PF08448: PAS_4" amino acids 261 to 359 (99 residues), 37 bits, see alignment E=1e-12 PF13426: PAS_9" amino acids 265 to 358 (94 residues), 32.3 bits, see alignment E=3.1e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 367 to 524 (158 residues), 105 bits, see alignment E=3.4e-34 PF00990: GGDEF" amino acids 370 to 520 (151 residues), 139.3 bits, see alignment E=3e-44 PF00563: EAL" amino acids 541 to 774 (234 residues), 241 bits, see alignment E=3.6e-75

Best Hits

KEGG orthology group: None (inferred from 67% identity to avn:Avin_07910)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIV8 at UniProt or InterPro

Protein Sequence (797 amino acids)

>Psest_0651 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MLTSLSLRKSMPLLLGLFGLLFTVLLMLWHLPTRIEETLTGWRNHTHQHLSLLQSSLSDH
RRLGRVAELETELADLASLEGVRWAMVIDPEFKVIAATRLGLAPERLELIRADDLAALVA
SGRAGWIGEDADQQLAIYPLDRVAQNGTPLREALLVEYDFAPMLAQTKREAWFYLAQTLA
LLLLLGLILNRLYGRLLTRRLACIDQAARQFALDQQPQPPAVSGRDEIGKLALTLSRMMN
QLHERQLAVSESERLFRELVDTAPIGMLVVDSDLRVLQANPAAAALFGCSPDELSNNLPG
DRLVEGDATARLRSTPANTTLELTGRRNGQEVPLEISCTPFQRYGEKHFLVLLRDISDRL
RAEQRLRFLAHFDPLTQLANRNYLMQRLEQLLSARLPLSLLYLDLDHFKRINDSLGHEVG
DKLLVQIGDRLGQLMPEHALLSRTGGDEFMLLLDGCDIASAEQLARRIIDGFQPPVRIGQ
YECFVSPSIGITVSDGRHSASDLLKQVDLALYAAKDAGRNCTVVYNEALSEAAAGRRALE
QALRRALDRDEFVLHYQAQVNAQGEPEVMEALLRWNSPERGLVPPGEFIPVLEDSGMIIE
ATRWVFREACRQACRWHAQGYSLRIAVNLSPLDFRQADLAGSLLAILEEEGASTSMLELE
ITESALLEADEHVQQTLTRLKAAGLPLLLDDFGTGYASLTYLQRFRFDGIKIDREFVSGL
PDSEESVALVRGILTMASHLGLHVVAEGVENERQAAFLRLNGCPSLQGFYYARPQPAAQC
RLQQNGGPINLQLPLDA