Protein Info for Psest_0629 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 841 TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 839 (836 residues), 1220.1 bits, see alignment E=0 PF00270: DEAD" amino acids 15 to 166 (152 residues), 34 bits, see alignment E=6.2e-12 PF00271: Helicase_C" amino acids 209 to 333 (125 residues), 50 bits, see alignment E=8.3e-17 PF04408: HA2_N" amino acids 398 to 422 (25 residues), 21.3 bits, see alignment (E = 5.7e-08) PF24473: CON_HrpB" amino acids 594 to 628 (35 residues), 35.4 bits, see alignment (E = 1.9e-12) PF08482: HrpB_C" amino acids 696 to 827 (132 residues), 183 bits, see alignment E=7.6e-58

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 81% identity to pfl:PFL_5417)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GER3 at UniProt or InterPro

Protein Sequence (841 amino acids)

>Psest_0629 ATP-dependent helicase HrpB (Pseudomonas stutzeri RCH2)
MSLLPIESVLPALRDALSARNEVVLEAAPGAGKTTRVPIALLEEPWLADQTIVMLEPRRL
AARAAAERLASELGESVGQTVGYRIRLESKVGPQTRIEVVTEGILTRRLQDDPALDGVGL
LIFDEYHLRSLDADLALALCLNGRELLRDEPPLKVLLMSATLEGERLSRLLDEAPVVRSE
GRMYPVEQRWGRPSQIGEALEPRVVQTMLQALADEPGSLLVFLPGQAEIRRVAEQLAERL
AGRSEILLCPLHGELDLAAQRAAIEPAPAGKRKVVLATNIAETSLTIDGVRVVVDAGLER
VPRFDPASGMTRLDTQRISRSSATQRAGRAGRLQPGACYRLWSEAQHEQLAAHGSAEILQ
ADLAGLALQLARWGIGDPNELAWLDPPPTAAYAQGRDLLQRLGALADDGNLTRHGQAMAE
LPAHPRIAHLLLRGHALGLGGLACDLAALLGERDILRGGGADLHSRLALLAGNDKAARGA
RGGVQRARQLARQFRSYLRGPASEPVADPDHPRWLGCLLAFAYPDRIAQQRRAGGADYRL
ANGRAATFGEPDALMKEPWLVIADLGSRQGQREERIYLAADLDPALFDGPLAEQVRCQDL
LDWEEREGVLRAERQVKVGELVLAREALAELDDEARSRALLGLVRRKGLELLPWSAELRQ
WQARVALLRRLDLADTGSSEWPDLSDAALLASLEEWLQPWLGKVSRLSHFANLDLAGILH
GLLPWPLPQRLDELAPRTLEVPSGSRIRLDYSDEMPVLAVRLQELFGLADTPRIAGGRQG
VKLHLLSPAQRPVQVTQDLASFWRNTYAEVKKDLRGRYPKHYWPEDPLVAQATARAKPRK
S