Protein Info for Psest_0610 in Pseudomonas stutzeri RCH2

Annotation: heavy metal sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 160 to 184 (25 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 3 to 454 (452 residues), 441.3 bits, see alignment E=2.3e-136 PF00672: HAMP" amino acids 182 to 234 (53 residues), 37.6 bits, see alignment 3.4e-13 PF00512: HisKA" amino acids 240 to 302 (63 residues), 52.4 bits, see alignment E=6.7e-18 PF02518: HATPase_c" amino acids 350 to 456 (107 residues), 77.5 bits, see alignment E=1.6e-25

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 48% identity to pfl:PFL_3148)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGS3 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Psest_0610 heavy metal sensor kinase (Pseudomonas stutzeri RCH2)
MKPLSLASRLALQITLTGAALVALLIALSYWVLVRQLELRAQEEVTAKLSQIDHGLLEDT
KARMGRSWQHALSDTVLGHDNLSITVIGDTAKSPIFSIGRFANAPQQLDLVSRDGDYLGW
TTKDGVQMLTGRKQIQVPGLAPMTLLLSQDRSADQRLVAAFLRSALVTVPMLLILIGLAG
WLIAQNGLRPLRKFRALATKVSTQDLSPRIRTDRLPQELQALAHSLNVMLHRLDDGVQQL
SQFSDDVAHELKTPLNNLIGKAQVTLVRERSKEHYREVIESSVEELERMDRIVSDMLFLA
QASHASPALKLEQLSLGSEARRVCEYFEVLAEEAGVATTVTGDAMILGNRLMVQRAISNL
LSNALRHSNTGSTVELKILEEDVDIVSLAVTNHGATIESDHLPHLFDRFYRVNGIQPRGA
GLGLAIVRSVMNLHKGHVAVASKDGRTTFELTFPRQA