Protein Info for Psest_0600 in Pseudomonas stutzeri RCH2

Annotation: copper-(or silver)-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 787 transmembrane" amino acids 136 to 159 (24 residues), see Phobius details amino acids 166 to 188 (23 residues), see Phobius details amino acids 198 to 222 (25 residues), see Phobius details amino acids 235 to 253 (19 residues), see Phobius details amino acids 388 to 410 (23 residues), see Phobius details amino acids 416 to 438 (23 residues), see Phobius details amino acids 732 to 754 (23 residues), see Phobius details amino acids 760 to 779 (20 residues), see Phobius details PF04945: YHS" amino acids 20 to 62 (43 residues), 58.3 bits, see alignment 1.6e-19 PF19335: HMBD" amino acids 91 to 116 (26 residues), 40.7 bits, see alignment (E = 4.4e-14) TIGR01511: copper-translocating P-type ATPase" amino acids 179 to 782 (604 residues), 584.7 bits, see alignment E=4.3e-179 TIGR01525: heavy metal translocating P-type ATPase" amino acids 198 to 781 (584 residues), 602.7 bits, see alignment E=1.7e-184 TIGR01494: HAD ATPase, P-type, family IC" amino acids 242 to 766 (525 residues), 282.9 bits, see alignment E=7.6e-88 PF00122: E1-E2_ATPase" amino acids 271 to 451 (181 residues), 195.2 bits, see alignment E=1.8e-61 PF00702: Hydrolase" amino acids 469 to 686 (218 residues), 109.8 bits, see alignment E=5.8e-35

Best Hits

Swiss-Prot: 55% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 74% identity to pmy:Pmen_2229)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GEP2 at UniProt or InterPro

Protein Sequence (787 amino acids)

>Psest_0600 copper-(or silver)-translocating P-type ATPase (Pseudomonas stutzeri RCH2)
MSSQQCHHQPDQTKGTAALTDPVCGMKVEEDSEHQEHYQGSTYRFCSQGCQTKFRSDPAR
YLAAPQAHGTSSRASTPPAQKPPAGDATTEYTCPMHPEIRQMGPGDCPICGMSLEPLIPE
LDEEENPELKDFSKRFWWSLPLTVAVTLLAMAGHAIPLFHGASQNWVELALTTPVVLWAG
WPFFARGLASVKHRSPNMWTLIGLGTGAAYIYSVVATLLPSMFPESFTVGGRIGVYYEAA
AVIISLTLLGQLLELKARSQTSSAIKSLLGLSPKTARRIAKDGSEEDIPLTHVHEGDHLR
VRPGEKVPVDGEVLEGESAVDESMLTGEPVPVTKRTGDSLIGATMNTSGSLVMRATRVGS
GTMLSQIVQMVANAQRSKAPMQRMADTVAGYFVLTVIGIALLTFFAWGLFGPDQGWVFGL
INAVAVLIIACPCALGLATPMSIMVATGKAAGSGVLFRDAGAIENVRKVDTLIVDKTGTL
TEGRPVFDRAIGVTPFDSQEVIRLSASLDQGSEHPLAHAIVDHARSEGLQLAKPETFESG
SGIGVRGLVEGKQLQLGNTALMEDAGVSVEPLKYQAEKMRESGTSIVYLAVDGALAGLLA
VSDPIKPTSKEAVARLQAEGVQVIMATGDGLTTARAVARELSLDEVHGEVKPQDKEALVV
KLQALGKVVAMAGDGINDAPALARADVGIAMGTGTDVAMNSAQVTLVKGDLMGILRARTL
SVATVRNMRQNLLFAFMYNGLSIPIAAGLLYPFFGLLLSPMIAALAMSLSSASVVFNALR
LRQTRVV